阿尔茨海默病并发HSV-1感染患者外周血BDNF与NGF和CD31水平

Peripheral blood BDNF,NGF and CD31 of Alzheimer’s disease patients with HSV-1 infection

目的 探讨阿尔茨海默病并发单纯疱疹病毒-1(HSV-1)感染患者外周血脑源性神经营养因子(BDNF),神经营养因子(NGF),血小板-内皮细胞黏附分子(CD31)水平。方法 回顾性分析2017年8月-2020年9月河南省濮阳市人民医院神经内科收治的86例阿尔茨海默病患者一般临床资料,将HSV-1感染者纳入感染组(n=65),将未出现HSV-1感染者纳入对照组(n=21),分析两组外周血BDNF、NGF、CD31与载脂蛋白E(ApoE)、tau、淀粉样β(Aβ)蛋白及氧化应激指标的相关性。结果 感染组BDNF、NGF、CD31均低于对照组(P<0.05);感染组脑脊液中ApoE、tau、Aβ蛋白水平均高于对照组(P<0.05);感染组SOD、MDA、NOS水平均高于对照组(P<0.05);BDNF、NGF、CD31与ApoE、tau、Aβ蛋白、SOD、MDA、NOS呈负相关(P<0.05);感染组Cog-12评分高于对照组,MMSE、ADL评分低于对照组(P<0.05)。结论 HSV感染促进AD发生、发展,会降低脑脊液中DNF、NGF、CD31水平,可能与改变AD患者ApoE、tau、Aβ蛋白,诱导氧化应激反应有关,且感染后患者认知功能、精神状态及生活质量均降低。

OBJECTIVE To investigate the effect of herpes simplex virus-1(HSV-1) infection on the expression of peripheral blood brain-derived neurotrophic factor(BDNF),neurotrophic factor(NGF) and platelet/endothelial cell adhesion molecule-1(CD31) in patients with Alzheimer’s disease(AD).METHODS The general clinical data of 86 patients with AD admitted to the Puyang People’s Hospital between Aug.2017 and Sep.2020 were retrospectively analyzed.Patients with HSV-1 infection were included in the infection group(n=65),and those without HSV-1 infection were included in the control group(n=21),and the correlation between peripheral blood BDNF,NGF,CD31,BDNF,NGF,CD31,ApoE,tau,Aβ protein,and oxidative stress indicators in both groups was analyzed.RESULTS BDNF,NGF,and CD31 in the infection group were significantly lower than those in the control group(P<0.05).The levels of ApoE,tau,and Aβ protein in cerebrospinal fluid of the infection group were significantly higher than those in the control group(P<0.05).The levels of SOD,MDA,and NOS in the infection group were significantly higher than those in the control group(P<0.05).BDNF,NGF and CD31 were negatively correlated with ApoE,tau,Aβ protein,SOD,MDA and NOS(P<0.05).The infection group had significantly higher Cog-12 scores,and significantly lower MMSE and ADL scores,compared with the control group(P<0.05).CONCLUSION HSV-1 infection promoted the occurrence and development of AD,and decresed the levels of DNF,NGF and CD31 in cerebrospinal fluid,which might be associated with the alteration of ApoE,tau and Aβ proteins,and the induction of oxidative stress in patients with AD.Moreover,the cognitive function,mental state and quality of life of the patients was significantly reduced after infection.