ApoE和IL-8基因多态性与儿童肺炎支原体肺炎易感性的关联

Association of ApoE and IL-8 gene polymorphisms with susceptibility Mycoplasma pneumoniae pneumonia in children

目的 分析载脂蛋白E(ApoE)、白细胞介素-8(IL-8)基因多态性与儿童肺炎支原体肺炎感染的关联性。方法 回顾性分析2018年8月-2021年8月重庆两江新区第一人民医院收治的156例肺炎支原体肺炎(MPP)患儿的临床资料(设为MPP组),并纳入经性别、年龄1∶1匹配的156名健康体检患儿作为对照组,比较两组ApoE基因rs7412、rs429358位点及IL-8基因rs4073、rs2227306位点基因型及基因频率分布,并分析ApoE及IL-8基因多态性与MPP患儿支气管黏膜形态学改变及预后转归的关系。结果 Hardy-Weinberg平衡检验提示各基因频率符合遗传平衡法则,具有较好群体代表性;MPP组ApoE基因rs429358位点CC型及等位基因C频率高于对照组(P<0.05),CC型患MPP的OR=3.694,携带等位基因C患MPP的OR=1.849;MPP组IL-8基因rs4073位点AA型及等位基因A频率高于对照组(P<0.05),AA型患MPP的OR=4.104,携带等位基因A患MPP的OR=1.457;ApoE基因rs429358位点及IL-8基因rs4073位点不同基因型支气管黏膜形态、预后转归时间差异均有统计学意义(P<0.05),其中ApoE rs429358位点CC基因型及IL-8 rs4073位点AA基因型黏液栓阻塞型表现最多(P<0.05),且退热时间、咳嗽消失时间、肺部啰音消失时间、住院时间更长(P<0.05)。结论 ApoE rs429358及IL-8 rs4073位点多态性与MPP易感性及患儿支气管黏膜形态、预后转归情况均有关,有望成为MPP诊疗的新靶点。

OBJECTIVE To analyze the association of apolipoprotein E(ApoE) and interleukin-8(IL-8) gene polymorphisms with Mycoplasma pneumoniae pneumonia in children.METHODS The clinical data of 156 children with Mycoplasma pneumoniae pneumonia(MPP)(set as MPP group) between Aug.2018 and Aug.2021 in the First People′ s Hospital of Chongqing Liangjiang New Area were retrospectively analyzed,and 156 children with healthy physical examination matched by gender and age at 1∶1 were included as control group.The distribution characteristics of genotypes and gene frequencies at rs7412 locus and rs429358 locus of ApoE gene and at rs4073 locus and rs2227306 locus of IL-8 gene were compared between the two groups.The relationship between ApoE and IL-8 gene polymorphisms and bronchial mucosal morphological changes and prognosis and outcomes in children with MPP was analyzed.RESULTS The Hardy-Weinberg equilibrium test suggested that the frequencies of each gene were consistent with the law of genetic equilibrium and had a good population representation.The frequencies of CC genotype and allele C at rs429358 locus of ApoE gene in MPP group were higher than those in control group(P<0.05).The relative risk OR of MPP in patients with CC genotype was 3.694,and the OR of MPP in patients carrying allele C was 1.849.The frequencies of AA genotype and allele A at rs4073 locus of IL-8 gene were higher in MPP group than those in control group(P<0.05),and the OR of MPP in patients carrying AA genotype was 4.104 and the OR of MPP in patients carrying allele A was 1.457.There were significant differences in bronchial mucosal morphology and prognostic outcome time of different genotypes at rs429358 locus of ApoE gene and at rs4073 locus of IL-8 gene(P<0.05).The type of CC genotype at rs429358 locus of ApoE gene and AA genotype at rs4073 locus of IL-8 gene with mucus plug obstruction were the most(P<0.05),and the fever abatement time,cough disappearance time,pulmonary rales disappearance time and hospital stay were longer(P<0.05).CONCLUSION The polymorphisms at rs429358 locus of ApoE gene and at rs4073 locus of IL-8 gene were associated with the MPP susceptibility,the morphology of bronchial mucosa and the prognosis and outcomes of children,which were expected to be new targets for the diagnosis and treatment of MPP.