摘要
目的:观察乳腺癌并发抑郁症(BCRD)模型小鼠行为、白介素类细胞因子的变化。方法:BALB/c雌性小鼠,腋下接种1×106个4T1炎症乳腺癌细胞,7 d后观察是否成模,成模后的小鼠连续21 d背部皮下注射皮质酮(30 mg/kg)复制BCRD模型。所有小鼠按糖水消耗随机分为4组:乳腺癌组、抑郁症组、BCRD组、空白对照组。21 d后,测定各组小鼠糖水偏嗜度、旷场实验等行为学变化;采用悬液芯片技术检测血清、海马、肿瘤、皮质组织中IL-1β、IL-2、IL-6、IL-10、IL-12含量。结果:与空白组比较,BCRD小鼠蔗糖偏嗜度及垂直活动次数降低(P<0.01或P<0.05),血清、海马中IL-2、IL-6、IL-12含量显著升高(P<0.01或P<0.05),海马中IL-10含量显著增加(P<0.01或P<0.05);与乳腺癌组比较,BCRD组血清、海马、皮质中IL-6含量显著升高(P<0.05),与抑郁症组比较,血清、海马、皮质中IL-6含量差异无统计学意义(P>0.05),但均有升高趋势。结论:IL-6在血清、海马、皮质中表达均较高且均一,可能是BCRD的重要标志物。
Objective:To observe the changes of behavior and interleukin-cytokines in mice with breast cancer related with depression(BCRD).Methods:The female BALB/c mice were inoculated with 1×1064T1 inflammatory breast cancer cells under the armpit.After 7 days,the mice were injected subcutaneously with corticosterone(30 mg/kg)in the back for 21 days to replicate the BCRD model.All mice were randomly divided into four groups according to the consumption sugar water:breast cancer group,depression group,BCRD group,and normal group.After 21 days,the behavioral changes of mice in each group,were measured.such as sugar water preference and open field experiment,The contents of IL-1β,IL-2,IL-6,IL-10 and IL-12 in serum,hippocampus,tumor and cortex were measured by suspension chip technology.Results:Compared with normal group,the sucrose preference degree,horizontal and vertical activity times of BCRD mice decreased(P<0.01 or P<0.05),the contents of IL-2,IL-6 and IL-12 in serum,hippocampus and cortex were increased significantly(P<0.01 or P<0.05),and the contents of IL-10 in hippocampus was increased significantly(P<0.01 or P<0.05).Compared with breast cancer group,the IL-6 content in serum,hippocampus and cortex in the BCRD group was significantly increased(P<0.05),and compared with the depression group,there was no significant difference in the IL-6 content in serum,hippocampus and cortex(P>0.05),but all showed an increasing trend.Conclusion:The expression of IL-6 in serum,tumor,hippocampus and cortex is high and uniform,which may be an important marker of BCRD.
作者
孟盼
罗薇絮
蔺晓源
刘检
赵洪庆
凌佳
骆妍
王宇红
MENG Pan;LUO Weixu;LIN Xiaoyuan;LIU Jian;ZHAO Hongqing;LING Jia;LUO Yan;WANG Yuhong(Science and Technology Innovation Center of Hunan University of Chinese Medicine,Changsha 410208,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2023年第2期276-280,共5页
Chinese Journal of Immunology
基金
湖南省自然科学基金优秀青年项目(2020JJ3027)
湖南省科技创新人才计划优秀博士后创新人才项目(2020RC2060)
长沙市杰出创新青年培养计划项目(kq2009018)
湖南省三尖计划青年科技人才项目(2022RC1226)。