红麻种质资源对根结线虫抗性鉴定与评价

Identification and Evaluation of Kenaf Germplasm Resistance to Root Knot Nematode

为鉴定我国红麻种质资源对根结线虫病的抗病性,本研究采用SCAR引物对侵染红麻的根结线虫种群进行了鉴定,并采用田间自然发病及盆栽接种鉴定的的方法,对220份红麻野生或栽培种质资源的根结线虫抗性进行了鉴定与评价。研究结果表明,侵染红麻的线虫种类为南方根结线虫(Meloidogyne incognita)。田间抗性鉴定结果显示高抗红麻资源有133份,占资源总数量的60.45%,玫瑰茄、红叶木槿和金钱吊芙蓉全部表现为高抗,高抗比率最高。盆栽接种鉴定结果显示免疫资源有7份,占资源总数的3.18%;高抗资源有98份,占资源总数的44.55%;玫瑰茄均鉴定为高抗等级。红麻种质在田间自然发病和盆栽发病抗性鉴定存在一定的差异,两种方法鉴定结果一致的种质资源共有98份,其中高抗资源69份,占交集资源数量的70.41%;高抗资源中有49份来源于栽培红麻,10份来源于野生近缘,其余来源于玫瑰茄、玫瑰麻和红叶木槿,1份高感品种来自于野生近缘。本研究结果为红麻种质资源发掘利用提供科学依据。

In order to identify kenaf germplasm resources resistant to root knot nematode,In this study,the root knot nematode population infected with kenaf was identified by SCAR primers,and the root knot nematode resistance of 220 kenaf wild or cultivated germplasm resource materials were identified and evaluated by using the methods of natural field disease and pot inoculation identification.The results showed that the pathogen causing disease in the field was Meloidogyne incognita.The results of field resistance identification showed that there were 133 kenaf resources showed high resistance,accounting for 60.45%,and all the roselle,false roselle and creeping saxifrage showed high resistance,and the high resistance ratio was the highest.The results of pot inoculation showed that there were 7 immune resources,accounting for 3.18%of the total resources.And 98 high resistance resources,accounting for 44.55%of the total resources.All roselle were identified as high resistance grade.There were some differences in the identification of kenaf germplasm resistance between natural field disease and pot test.There were 98 germplasm resources with the same identification results by the two methods.There were 69 high resistance resources,accounting for 70.41%of the 98 germplasm resources.Among the high-resistance resources,49 were from cultivated kenaf,10 were from wild relatives,the rest were from roselle,rose hemp and hibiscus,and 1 high susceptible variety was from wild relatives.These results of this study provided scientific basis for the exploitation and utilization of kenaf germplasm resources against root knot nematode disease.