不同植物生长调节剂处理对31、花叶、怀莱苹果砧木试管苗扩繁的影响

Effects of Different Plant Growth Regulator Treatment on Propagation of 31,Mosaic and Huailai Apple Rootstock Test-tube Seedlings

【目的】以3个苹果砧木为试材,研究了不同外源激素的浓度配比对苹果砧木试管苗扩繁的影响,为苹果砧木试管苗繁殖效率的提高提供理论基础。【方法】以MS作为基本培养基,以31、花叶、怀莱为试验材料,研究了不同浓度的BA以及NAA对试管苗扩繁的影响。【结果】添加适宜浓度的生长调节剂可以显著提高试管苗的扩繁系数,促进其生长;其中适用于31试管苗继代培养的生长调节剂为MS培养基+BA 4 mL/L+NAA 3 mL/L,其扩繁系数达4.75,株高为2.03 cm;适宜花叶试管苗继代培养的生长调节剂为MS培养基+BA 3 mL/L+NAA 3 mL/L组合,其扩繁系数为5.8,株高达1.53 cm;当生长调节剂浓度为MS培养基+BA 4 mL/L+NAA 2 mL/L时,怀莱试管苗的扩繁系数达最大为2.8,株高为1.64 cm。【结论】不同生长调节剂浓度组合对苹果砧木试管苗扩繁的影响不同。

【Objective】With three apple rootstock as test materials,the effects of different concentrations of exogenous hormones on the propagation of apple rootstock test-tube seedlings were studied to provide a theoretical basis for improving the propagation efficiency of apple rootstock test-tube seedlings.【Methods】In this experiment,MS was used as the basic medium,and 31,Mosaic and Huailai were used as experimental materials to study the effects of different concentrations of BA and NAA on the propagation of test-tube seedlings.【Results】The test results showed that adding a suitable concentration of growth regulators could significantly improve the propagation coefficient of test-tube seedlings and promote their growth;among them,the growth regulators suitable for the subculture of 31 test-tube seedlings were MS medium+BA 4 mL/L+NAA 3 mL/L.Its propagation coefficient was 4.75,and the plant height was 2.03 cm;the growth regulator suitable for the subculture of mosaic test-tube seedlings was the combination of MS medium+BA 3 mL/L+NAA 3 mL/L.Its propagation coefficient was 5.8,and the plant height was 1.53 cm.When the concentration of growth regulator was MS medium+BA4 mL/L+NAA2 mL/L,the propagation coefficient of Huailai test-tube plantlets reached the maximum of 2.8,and the plant height was 1.64 cm.【Conclusion】Different growth regulator concentration combinations have different effects on the propagation of apple rootstock test-tube seedlings.