miR-1181在鼻咽癌组织中的表达及其临床意义

Expression of miR-1181 in Nasopharyngeal Carcinoma and its Clinical Significance

【目的】探讨微小RNA-1181(miR-1181)在鼻咽癌(Nasopharyngeal carcinoma,NPC)组织中的表达及其临床意义。【方法】连续随机招募2019年10月至2021年5月在安康市中心医院接受手术治疗的71例NPC患者,采用实时荧光定量PCR(RT-PCR)检测miR-1181在NPC组织和癌旁正常组织中的相对表达量,比较不同分化程度、临床分期、是否远处转移患者NPC组织中miR-1181的相对表达量。对所有患者术后进行随访,分析miR-1181与NPC预后的相关性。【结果】与癌旁正常组织相比,miR-1181在NPC组织中显著下调;miR-1181在临床分期Ⅲ期+Ⅳ期NPC组织中相对表达量低于Ⅰ期+Ⅱ期,在中低分化NPC组织中miR-1181相对表达量低于高分化组织,在发生远处转移的NPC组织中miR-1181相对表达量低于未转移组织,差异均有统计学意义(P<0.05)。预后良好患者miR-1181相对表达量显著高于预后不良患者(P<0.05);Logistic回归分析结果显示:miR-1181<3.59与NPC预后不良相关,其优势比(OR)(95%CI)为3.52(1.65~5.28)。【结论】miR-1181在NPC组织中呈低表达,在高分化、临床分期低及发生远处转移的NPC组织中高表达,且miR-1181<3.59是NPC患者预后不良的危险因素。

【Objective】To investigate the expression and clinical significance of microRNA-1181(miR-1181)in nasopharyngeal carcinoma(NPC).【Methods】A total of 71 NPC patients who received surgical treatment in the Ankang Central Hospital from October 2019 to May 2021 were randomly recruited.The relative expression of miR-1181 in NPC tissues and normal tissues adjacent to cancer was detected by real-time fluorescent quantitative PCR(RT-PCR),and the relative expression of miR-1181 in NPC tissues of patients with different degrees of differentiation,clinical stages,and distant metastasis was compared.All patients were followed up to analyze the correlation between miR-1181 and the prognosis of NPC.【Results】Compared with normal tissues adjacent to cancer,miR-1181 was significantly down-regulated in NPC tissues;The relative expression of miR-1181 in clinical stageⅢ+ⅣNPC tissues was lower than that in stageⅠ+ⅡNPC tissues,the relative expression of miR-1181 in moderately and poorly differentiated NPC tissues was lower than that in highly differentiated NPC tissues,and the relative expression of miR-1181 in NPC tissues with distant metastasis was lower than that in non-metastatic NPC tissues,the difference was statistically significant(P<0.05).The relative expression of miR-1181 in patients with good prognosis was significantly higher than that in patients with poor prognosis(P<0.05);Logistic regression analysis showed that miR-1181<3.59 was associated with poor prognosis of NPC,and its odds ratio(OR)(95%CI)was 3.52(1.65~5.28).【Conclusion】miR-1181 is low expression in NPC tissues,and high expression in NPC tissues with high differentiation,low clinical stage and distant metastasis,and miR-1181<3.59 is a risk factor for poor prognosis of NPC patients.