响应面法优化紫米糠蛋白质的提取工艺

Optimization of protein extraction process from purple rice bran by response surface methodology

以紫米糠为原料,比较碱法、超声波辅助碱法、碱性蛋白酶法和纤维素酶法提取紫米糠蛋白的提取率,选择超声波辅助碱法进行单因素试验,采用Box-Behnken试验优化紫米糠蛋白提取工艺。结果表明:最佳提取条件为超声功率200 W、超声时间22 min、料液比1∶23(g/mL),在此条件下紫米糠蛋白提取率为90.58%±0.32%.

With purple rice bran as raw material,the extraction rate of purple rice bran protein by alkali method,ultrasonic assisted alkali method,alkaline protease method and cellulase method were compared.The ultrasonic assisted alkali method was selected for single factor test.Box-Behnken test was used to optimize the extraction process of purple rice bran protein.The results showed that the optimum extraction conditions were ultrasonic power 200 W,ultrasonic time 22 min,and the ratio of material to liquid 1∶23(g/mL).Under these conditions,the extraction rate of purple rice bran protein was 90.58%±0.32%.