高效液相色谱-串联质谱法同时测定蜜饯中8种甜味剂

Simultaneous Determination of Eight Sweeteners in Candied Fruit by High Performance Liquid Chromatography-Tandem Mass Spectrometry

目的:建立蜜饯中8种甜味剂高效液相色谱-质谱法。方法:蜜饯样品经0.1%甲酸水超声提取后上质谱分析仪进行外标法含量分析。采用C18色谱柱进行分离,流动相为0.1%甲酸-甲醇,梯度洗脱。结果:8种甜味剂在2~5600 ng·mL^(-1)呈线性关系,蜜饯的加标回收率为85.0%~106.1%,相对标准偏差均小于8.0%。结论:应用此方法对蜜饯中的8种甜味剂含量进行检测,简单快速,准确度和灵敏度高,大大缩短了检测时间和检验样品用量。

Objective:To establish high performance liquid chromatography-mass spectrometry for 8 kinds of sweeteners in preserved fruit.Method:The preserved fruit samples were extracted with 0.1%formic acid and analyzed by external standard method with mass spectrometry.The separation was carried out on a C18 column with 0.1%formic acid-methanol as mobile phase and gradient elution.Result:The results showed that there was a linear relationship between the eight sweeteners at 2~5600 ng·mL^(-1),and the recovery rate of added standard was 85.0%~106.1%,and the relative standard deviation was less than 8.0%.Conclusion:Using this method to detect the content of eight sweeteners in candied fruits is simple,fast,accurate and sensitive,which greatly reduces the detection time and the amount of test samples.