茶树WRKY基因家族特征及生物胁迫相关成员的生信分析

Comparative Analysis of Tea Plant WRKY Gene Family and Bioinformatic Analysis on the Members Related to Biotic Stresses

WRKY作为重要的植物转录因子,参与植物发育、代谢以及对生物和非生物胁迫的响应。然而,目前对生物胁迫下茶树中大多数WRKY成员的作用仍知之甚少。本研究利用生物信息学方法分别在茶树中国种和阿萨姆种的基因组中确定77和61个WRKY成员。根据结构域序列,中国种CssWRKY和阿萨姆种CsaWRKY家族成员均分为三组,第一组各有14和12个成员,第二组各有49和45个成员,第三组分别是14和4个成员。对基因结构、基序和表达特征的分析表明,两个茶树种的WRKY成员在内含子分布和基序组成上有极大的相似性,且在不同生物胁迫下的表达特征均表现较大差别。进一步预测WRKY的互作蛋白和靶标基因,结果表明生物胁迫下WRKY的互作蛋白参与“植物型过敏反应”和“茉莉酸介导的信号途径调控”等生物过程,靶标基因是防御反应或特定代谢途径中的重要分子。这些结果将为研究茶树WRKY在生物胁迫适应中的功能提供线索。

As an important transcription factor in plants, WRKY participated in the regulation of plant development, metabolism, and responses to biotic and abiotic stresses. However, the roles of most WRKY members in tea plant under biotic stress were still poorly understood. In the present study, 77 CssWRKY genes and 61 CsaWRKY genes were respectively identified in the genome of tea plant varieties Camellia sinensis var. sinensis and Camellia sinensis var. assamica using bioinformatic methods. Based on domain sequences, CssWRKY and CsaWRKY genes were both classified into three main groups with 14 and 12 members in GroupⅠ, 49 and 45 members in GroupⅡ,14 and 4 members in Group Ⅲ, respectively. Further analysis on gene-structure, motifs and expression profiles revealed that WRKY members in the two varieties showed great similarity in intron distribution and motif composition, and exhibited different expression patterns under biotic stresses. Moreover, the potential WRKYinteracting proteins and target genes under biotic stresses were predicted. GO enrichment analysis indicated that GO terms, ’plant-type hypersensitive response’ and ’regulation of jasmonic acid mediated signaling pathway’ were specifically enriched by WRKY interactors under anthracnose infe ction and pest attack. The target genes were involved in the pathways related to plant defense response and in some specific metabolic pathways. These results would provide novel insights into the function of tea plant WRKY in biotic stress adaptation.