HPLC同时测定苍耳子中4个化学成分的含量

Simultaneous Determination of Four Chemical Constituents in Fructus xanthii by HPLC

目的建立高效液相色谱测定苍耳子中绿原酸、咖啡酸、噻嗪二酮、咖啡酰基-噻嗪二酮苷4种化学成分的方法。方法采用Kromasil C_(18)色谱柱(5μm,4.6×250 mm),以甲醇-0.1%磷酸水溶液(20∶80;35∶65;v/v)为流动相,梯度洗脱,流速1 mL·min^(-1),柱温箱温度35℃,检测波长320nm;进样体积20μL。结果4种成分在各自的线性范围内线性关系良好,加样回收率在97.24~100.40之间,RSD分别为0.69%、1.47%、0.99%、1.56%。绿原酸、咖啡酸、噻嗪二酮、咖啡酰基-噻嗪二酮苷的标准曲线方程分别为Y=47575x+1077、Y=78601x+26.699、Y=9711.7x+13.411、Y=22052x+2.3687,在0.100~0.625 mg·mL^(-1)、0.02~0.100 mg·mL^(-1)、0.020~0.080 mg·mL^(-1)、0.020~0.070 mg·mL^(-1)浓度范围内线性关系良好,平均加样回收率RSD分别为0.69%、1.47%、0.99%、1.56%。结论建立的含量测定分析方法重复性好,稳定性强,灵敏度高,可用于苍耳子中4种成分的含量测定,为质量控制奠定基础。

OBJECTIVE A method for the determination of chlorogenic acid,caffeic acid,thiazidedione,and caffeoyl-thiazidedione glycosides in Fructus xanthii by HPLC was established.METHODS Kromasil C_(18)column(5μm,4.6×250 mm),mobile phase methanol-0.1%phosphoric acid aqueous solution(20∶80;35∶65;v/v),flow rate 1 mL·min^(-1),detection wavelength 320 nm,column oven temperature 35℃,injection volume 20μL.RESULTS The linear relationship of the 4 constituents was good in their respective linear range.The recoveries were 97.24~100.40%and the RSD were 0.69%,1.47%,0.99%and 1.56%respectively.The standard curve equations of chlorogenic acid,caffeic acid,buprofezin and caffeoyl-buprofezin were Y=47575x+1077,Y=78601x+26.699,Y=9711.7x+13.411,Y=22052x+2.3687,respectively.The linear relationship was good in the concentration range of 0.100-0.625 mg·mL^(-1),0.02-0.100 mg·mL^(-1),0.020-0.080 mg·mL^(-1),0.020-0.070 mg·mL^(-1).The average recovery RSD were 0.69%,1.47%,0.99%,1.56%.CONCLUSION The method was repeatable,stable and sensitive,and could be used for the determination of the 4 constituents in Fructus xanthii.