改良硬化剂注射法大鼠椎动脉颈椎病模型建立的实验研究

Experimental study of improved sclerotherapy injection in rat model of vertebral arteriocervical spondylopathy

目的:对硬化剂注射法制作法制作椎动脉颈椎病(cervical spondylosis of vertebral artery type,CSA)大鼠模型进行改良,评价改良硬化剂造模法制作大鼠CSA模型的有效性。方法:健康SPF级SD大鼠40只,雌雄各半,随机分为空白组20只,模型组20只。模型组采用改良硬化剂造模法造模,造模4周后观察大鼠的一般情况,经颅多普勒超声(transcranial Doppler,TCD)检测大鼠椎动脉血流速度、搏动指数、阻力指数,旷场试验检测大鼠精神紧张度。结果:造模后1~2 d,模型组大鼠均出现不同程度蜷缩喜卧、颤抖、眯眼、眼分泌物增多等情况,实验过程中未出现大鼠脱落及死亡情况。模型组的平均血流速度比空白组低(P<0.05),模型组的搏动指数、阻力指数比空白组高(P<0.05),模型组大鼠精神紧张度较空白组明显增高,组间差异具有统计学意义。结论:改良硬化剂造模法可成功制备CSA大鼠模型,具有成功率高、稳定性高、死亡率低、操作简单等优势。

Objective To improve the rat model of cervical spondylosis of vertebral artery type(CSA)induced by injecting sclerosing agent.To evaluate the efficacy of injecting sclerosing agent to induce CSA.Methods Forty Health SPF SD rats(20 males and 20 females),were randomly divided into two groups:the model group(20)and the blank group(20).All the animals were followed up for 4 weeks for the observation of general situation,transcranial Doppler(TCD)detection of blood flow velocity,pulsatility index and resistive index of the vertebral artery,measurement of mental distress by open-field test.Results One to two days after establish the animal model,rats in the model group appeared apathetic with decreased autonomic activities,trembling,squinting,increased eye excrement,etc.,and no rats died during the experiment.The mean blood flow velocity of the model group was lower than that of the blank group(P<0.05),and the pulsatilit index and resistive index of the model group were higher than that of the blank group(P<0.05).The mental distress of the model group was significantly higher than that of the blank group.Conclusion The modified injection of sclerosing agent is a practical method to establish the rat model of CSA,with high success rate,high stability,low mortality and simple operation.