基于荧光法生物气溶胶监测仪计数效率评价及方法研究

Research on the Counting Efficiency Evaluation and Method of Bioaerosol Monitoring Instrument Based on Fluorescence Method

生物气溶胶监测仪是一类快速、实时监测空气中生物气溶胶的新兴仪器。为系统评价生物气溶胶监测仪的计数效率,开展了荧光法生物气溶胶监测仪总粒子和荧光粒子2个通道的计数效率评价。首先设计搭建了1套生物气溶胶监测仪计数效率评价装置,然后通过表面原子转移自由基聚合反应(SI-ATRP)制备了单分散、荧光性质稳定的荧光聚苯乙烯微球。采用制备的荧光微球雾化法发尘,模拟生物气溶胶环境,开展计数效率的评价,总粒子数和荧光粒子数计数效率分别为98.9%和98.1%,评价结果表明搭建的装置能满足荧光法生物气溶胶监测仪计数效率的评价需求,对生物气溶胶检测具有意义。

Bioaerosol monitor is a kind of new instrument for fast and real time monitoring of air bioaerosol.The study evaluated the counting efficiency of the total particle channel and fluorescent particle channel of bioaerosol monitor instrument.First,a set of evaluation device for counting efficiency of bioaerosol monitor was designed and built.Then,the monodisperse and stable fluorescent polystyrene microspheres were prepared by SI-ATRP method.Bioaerosol environment was generated by atomizing fluorescent particles and found that the counting efficiency of total particle number and fluorescence particle number was 98.9%and 98.1%,respectively.The results showed that the device can meet the needs of the evaluation of the counting efficiency of the fluorescent bioaerosol monitor.It provided a scientific method for systematically evaluating the counting efficiency of a bioaerosol monitor,and the result was of great significance to the detection of bioaerosols.