响应面优化两种葛根黄酮浸提萃取工艺及抗氧化生理活性研究

The Response Surface Method Optimized the Extraction Process and Antioxidant Activity of Two Pueraria Lobata Flavonoids

为充分比较并研究两个完全不同品系的葛根黄酮最佳水提工艺及其抗氧化活性,以两个完全不同品种葛根——“赣葛2号”和花叶葛为试验材料,采用单因素试验、Box-Behnken试验对两种葛根超声辅助水提条件进行优化,同时研究比较不同黄酮质量浓度浸提液及两种葛根黄酮浸提液之间抗氧化活性变化差异。结果显示:在相同单因素试验条件下,花叶葛黄酮得率高于“赣葛2号”;超声功率350 W,超声温度35℃、超声时间75 min、料液比1∶30 g/m L为“赣葛2号”超声辅助浸提黄酮最佳条件;超声功率350 W,超声温度45℃、超声时间45 min、料液比1∶30 g/m L为花叶葛超声辅助浸提黄酮最佳条件;在最佳条件下,两种葛根黄酮得率分别为3.66 mg/g、4.08 mg/g;两种葛根均表现出很好的·OH自由基抑制效果、DPPH自由基清除能力和总抗氧化能力,且随着质量浓度变化抗氧化活性变化规律明显,综合比较,花叶葛抗氧化活性优于“赣葛2号”。

In order to fully compare and study the best water extraction process and antioxidant activity of Pueraria lobata flavonoids from two completely different strains, two completely different varieties of Pueraria lobata-"gange No. 2" and pueraria lobata were used as experimental materials, and the ultrasonic assisted water extraction conditions of the two Pueraria lobata were optimized by single factor test and box Behnken test, At the same time, the difference of antioxidant activity between the extracts of different flavonoids and two kinds of Pueraria flavonoids was studied and compared. The results showed that under the same single factor test conditions, the yield of Flavonoids from Pueraria lobata was higher than that of "gange 2";Ultrasonic Power 350W, ultrasonic temperature 35℃, ultrasonic time 75min, material liquid ratio 1∶30g/m L are the best conditions for ultrasonic assisted extraction of Flavonoids from "gange No.2";Ultrasonic Power 350W,ultrasonic temperature 45 ℃, ultrasonic time 45min, material liquid ratio 1 ∶30g/m L were the best conditions for ultrasonic assisted extraction of Flavonoids from Pueraria lobata;Under the optimum conditions, the yields of two kinds of Pueraria flavonoids were 3.66mg/g and4.08mg/g respectively;Both Pueraria lobata showed good ·OH free radical inhibition effect, DPPH free radical scavenging capacity and total antioxidant capacity, and the antioxidant activity changed obviously with the change of mass concentration. Comprehensive comparison showed that the antioxidant activity of Pueraria lobata was better than that of "gange 2".