玉米秸秆作为制备生物絮凝剂培养基技术研究

Study on Straw as Medium for Preparation of Bioflocculant

本研究利用秸秆发酵液作为产絮菌培养基,接种复合微生物絮凝菌(Bacillus sp.CX15),对絮凝菌培养条件进行了优化实验。本研究验证了秸秆降解程度,并对菌株产生的还原糖含量进行测定。结果表明,该菌上清液还原糖活性高于菌悬液和发酵液,发酵液还原糖产出率为35%,适宜的秸秆降解菌接种量为1%,秸秆降解最优时间为6 d,接种适宜的底物秸秆量为3 g,利用获得的秸秆糖化液作为絮凝剂低成本产絮培养基,絮凝菌反应60 h后,发酵液中补加0.5 g/L氮源调节秸秆糖化液碳氮比,絮凝率表现为90.4%,对污水表现出絮凝效果,具有良好的应用前景。

In this paper,the straw fermentation broth was used as the medium for flocculating bacteria,inoculated with a compound microbial flocculant(Bacillus sp.CX15)and optimized the culture conditions for flocculating bacteria.The reducing sugar content of the supernatant of the bacteria was higher than that of the suspension and fermentation broth,and the reducing sugar yield of the fermentation broth was 35%,the appropriate inoculum amount of straw degrading bacteria was 1%,the optimal time for straw degradation was 6 d,and the inoculum amount of the appropriate substrate straw was 3 g.The straw saccharification broth was used as a low-cost flocculant production medium,and after 60 h of flocculation bacteria reaction,the fermentation broth was supplemented with 0.5 g/L nitrogen source to adjust the carbon to nitrogen ratio of the straw molasses.The flocculation rate was 90.4%,which showed an obvious flocculation effect on the wastewater and has good application prospects.