基于超高效液相色谱-飞行时间质谱技术对婴幼儿缺铁性贫血的血清代谢组学研究

Serum metabolomics of iron deficiency anemia in infants based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

目的探讨婴幼儿缺铁性贫血与非缺铁性贫血血清的差异代谢物,探索潜在的生物标志物。方法基于Acquity UPLC BEH C_(18)色谱柱(100 mm×2.1 mm,1.8μm),采用超高效液相色谱-飞行时间质谱技术对30例缺铁性贫血6~11月龄新生儿和30例非缺铁性贫血6~11月龄新生儿的血清进行非靶向代谢组学分析,运用主成分分析和正交偏最小二乘判别法分析两组血清的代谢物差异,根据正交偏最小二乘判别法变量重要度值(variable importance projection,VIP)>1筛选差异代谢物,使用KEGG数据库对差异物进行相关代谢通路分析。结果缺铁性贫血组与非缺铁性贫血组血清代谢谱具有差异,44个潜在生物标志物主要为脂质,结合通路分析,差异代谢物相关的代谢途径包括甘油磷脂代谢与鞘脂代谢。结论婴幼儿非缺铁性贫血与缺铁性贫血组在脂质代谢物存在差异,提示缺铁性贫血的发生和进展与脂质的代谢有关。

OBJECTIVE To explore the differential metabolites in the serum of infants with iron deficiency anemia(IDA)and non iron deficiency anemia,and to explore the potential biomarkers.METHODS Non-targeted metabolomics of 30 infants with iron deficiency anemia aged 6-11 months and 30 infants with non iron deficiency anemia aged 6-11 months were analyzed by ultra-high performance liquid chromatography with time of flight mass spectrometry using Acquity UPLC BEH C_(18)column(100 mm×2.1 mm,1.8μm).The differences of metabolites between the two groups were analyzed by principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA).Differential metabolites were screened according to OPLS-DA variable importance projection(VIP)>1.The related metabolic pathways involved in the markers were analyzed based on the KEGG database.RESULTS Differences in serum metabolic profiles between iron deficiency anemia group and non iron deficiency anemia group were observed.The 44 potential biomarkers were mainly lipids.Combined with pathway analysis,the metabolic pathways related to different metabolites included glycerophosphingolipid metabolism and sphingolipid metabolism.CONCLUSION There are differences in lipid metabolites between infants with non iron deficiency anemia and iron deficiency anemia,suggesting that the occurrence and progress of iron deficiency anemia are related to lipid metabolism.