电感耦合等离子体质谱法测定血铅实验影响因素

Influence factors of lead in whole blood in the German External Quality Assessment Scheme by ICP-MS

目的通过参加2018—2020年《德国外部质量评估计划》(G-EQUAS)全血中铅国际比对实验,探讨影响电感耦合等离子体质谱法测定血铅实验结果的因素。方法变换测定模式,比对样品稀释倍数、内标元素、同位素等实验条件,应用电感耦合等离子体质谱法测定G-EQUAS全血中铅,对测定结果进行分析。结果仪器的质谱干扰、基体效应、物理效应、内标的选择、同位素的选择均是影响电感耦合等离子体质谱法测定血铅结果准确性的因素。结论应用电感耦合等离子体质谱法进行血铅测定实验时应规避实验中可能影响结果的因素。

OBJECTIVE To investigate the influence factors of lead in whole blood in the German External Quality Assessment Scheme(G-EQUAS)biological materials by inductively coupled plasma mass spectrometry(ICP-MS)from 2018 to 2020.METHODS ICP-MS was used to determine lead in whole blood of G-EQUAS by optimizing the reaction gas mode,sample dilution ratio,internal standard element,isotope and so on,analyzed the result.RESULTS This study indicated that selection of internal standard elements,isotopes,matrix effect and physical effect may affect the accuracy of ICP-MS determination of lead in biological samples.CONCLUSION Avoided the factors that may affect the result when determining lead in whole blood by ICP-MS.