妊娠期糖尿病患者外周血NOX 5基因甲基化检测

DETECTION OF NOX5 GENE METHYLATION IN PERIPHERAL BLOOD OF PATIENTS WITH GESTATIONAL DIABETES MELLITUS

目的检测妊娠期糖尿病(gestational diabetes mellitus,GDM)患者外周血NOX 5基因甲基化状态,并分析其与GDM的相关性。方法以2020—2021年我院产科门诊确诊为GDM的孕妇67例为病例组,同期门诊口服糖耐量正常的健康孕妇87例为对照组,采用甲基化特异性PCR方法检测两组孕妇NOX 5基因甲基化状态,并根据NOX 5基因甲基化状态将病例组孕妇分为阳性亚组44例和阴性亚组23例,根据是否胎儿窘迫、是否巨大儿将154例新生儿分为窘迫组(6例)和非窘迫组(148例)、巨大儿组(11例)和非巨大儿组(143例),分析NOX 5基因甲基化状态与GDM孕妇一般资料及妊娠结局的相关性。结果病例组和对照组NOX 5基因甲基化阳性构成比分别为65.67%、40.23%,两组比较差异有显著性(χ^(2)=9.807,P<0.05),阳性亚组和阴性亚组比较孕妇年龄、孕前体质量指数(BMI)、收缩压、舒张压、空腹血糖差异无显著性(P>0.05)。胎儿窘迫组与非窘迫组、巨大儿组与非巨大儿组NOX 5基因甲基化阳性构成比比较差异无显著性(P>0.05)。结论GDM患者NOX 5基因易发生甲基化,但NOX 5基因甲基化状态与GDM孕妇年龄、孕前BMI、血压、空腹血糖无明显相关,与胎儿窘迫、巨大儿也无明显相关。

Objective To investigate the methylation status of the NOX 5 gene in peripheral blood of patients with gestational diabetes mellitus(GDM)and its association with GDM.Methods A total of 67 pregnant women who were diagnosed with GDM at the outpatient service of Department of Obstetrics in our hospital from 2020 to 2021 were enrolled as case group,and 87 healthy pregnant women with normal oral glucose tolerance test results during the same period of time were enrolled as control group.Methylation-specific PCR was used to determine the methylation status of the NOX 5 gene,and according to the methylation status of the NOX 5 gene,the pregnant women in the case group were further divided into positive subgroup with 44 women and negative subgroup with 23 women.According to the presence or absence of fetal distress or fetal macrosomia,154 neonates were divided into distress group with 6 neonates and non-distress group with 148 neonates,as well as fetal macrosomia group with 11 neonates and non-fetal macrosomia group with 143 neonates.The methylation status of the NOX 5 gene was analyzed in terms of its association with the general information and pregnancy outcome of the pregnant women with GDM.Results There was a significant difference in the constituent ratio of positive NOX 5 gene methylation between the case group and the control group(65.67%vs 40.23%,χ^(2)=9.807,P<0.05),and there were no significant differences between the positive subgroup and the negative subgroup in age,pre-pregnancy body mass index(BMI),systolic blood pressure,diastolic blood pressure,and fasting blood glucose(P>0.05).There was no significant difference in the constituent ratio of positive NOX 5 gene methylation between the distress group and the non-distress group and between the fetal macrosomia group and the non-fetal macrosomia group(P>0.05).Conclusion NOX 5 gene methylation is often observed in pregnant women with GDM,but the methylation status of the NOX 5 gene is not associated with the age,pre-pregnancy BMI,blood pressure,and fasting blood glucose of pregnant women with GDM,and it is also not associated with fetal distress and fetal macrosomia.