6种益生菌及其添加方式对发酵米酒品质的影响

Comparison of fermentation effects of six kinds of probiotics on rice wine

目的:探究不同益生菌及其添加方式对米酒制作效果的影响。方法:以糯米为原料,通过外源添加鼠李糖乳杆菌BV-77、发酵乳杆菌TSF-331、唾液乳杆菌AP-32、约氏乳杆菌MH-68、嗜酸乳杆菌TYCA-06、干酪乳杆菌CS-773酿制益生菌米酒,对比只添加糖化酶、同时添加糖化酶和酵母以及添加方式(益生菌麸曲和益生菌菌粉)对米酒酒精度、还原糖、总酸和感官质量的影响。结果:酵母对益生菌米酒的发酵有促进作用,可显著提高米酒酒精度和还原糖含量。利用发酵乳杆菌菌粉同时添加用量为200 U/g的糖化酶和0.04%的酵母,在30℃发酵3 d的米酒发酵效果最佳,酒精度和还原糖含量达到最高,分别为7.20%vol和270.00 mg/mL,总酸含量达到最低,为8.40 mL/L,酿造出的米酒口感较为浓郁。结论:发酵乳杆菌是6种益生菌中最适宜酿造米酒的益生菌。

Objective: This study aimed to investigate the effects of 6 kinds of probiotics and their different adding ways on the production of rice wine. Methods: The probiotic rice wine was prepared by adding Lactobacillus rhamnosus BV-77, Lactobacillus fermentans TF-331, Lactobacillus saliva AP-32, Lactobacillus johnsoni MH-68, Lactobacillus acidophilus TYCA-06 and Lactobacillus casei CS-773. To compare the effects of saccharifying enzyme alone, saccharifying enzyme and yeast at the same time and adding methods(probiotic bran koji and probiotic powder) on alcohol content, reducing sugar, total acid and sensory quality of rice wine. Results: Yeast can promote the fermentation of probiotic rice wine and significantly improve the alcohol content and reducing sugar content of rice wine. The rice wine was fermented at 30 ℃ for 3 days by adding 200 U/g saccharifying enzyme and 0.04% yeast, with the highest alcohol content and reducing sugar content of 7.20%vol and 270.00 mg/mL, respectively, and the lowest total acid content of 8.40 mL/L. The rice wine is relatively rich in taste. Conclusion: Lactobacillus fermentans is the most suitable probiotics for rice wine brewing among 6 kinds of probiotics.