AGTR1基因rs2638360位点多态性对中国人群缺血性脑卒中发生风险的影响

Prospective study on the effects of rs2638360 polymorphisms in AGTR1 on the risk of occurrence of ischemic cerebral stroke in Chinese population

目的评估血管紧张素Ⅱ-1型受体(angiotensinⅡreceptoRtype 1,AGTR1)基因rs2638360位点单核苷酸多态性(single-nucleotide polymorphism,SNP)与缺血性脑卒中(ischemic stroke,IS)发生风险的前瞻性关联。方法利用“135”前瞻性队列研究(2013至2019)的数据,对1569名无血缘关系的中国成年人进行rs2638360位点的分型。使用R语言3.6.2的SNPassoc包评估rs2638360位点突变与IS发生风险的相关性,使用SAS 9.4利用Cox比例风险模型进行分层分析及叉生分析。结果6年的随访期间,68人(4.33%)发生IS。未发现AGTR1基因rs2638360位点单核苷酸多态性影响IS的发生风险,但在年龄≥51岁或低密度脂蛋白胆固醇(LDL-C)<2.58 mmol/L的研究对象中,与携带rs2638360位点TT基因型的个体比较,携带该位点TC/CC基因型的个体发生IS的风险增加(P<0.05),且rs2638360位点单核苷酸多态性与年龄或高密度脂蛋白胆固醇(HDL-C)可产生联合作用显著影响IS的发生风险(P<0.05)。结论携带rs2638360位点TC/CC基因型且年龄≥51岁或LDL-C水平较低的个体可能更容易发生IS,且rs2638360位点单核苷酸多态性与年龄或HDL-C可产生联合作用,显著影响中国人群发生IS的风险。

Objective To investigate the prospective correlation between the single-nucleotide polymorphism(SNP)at rs2638360 locus of AGTR1 gene and the risk of occurrence of ischemic stroke(IS)in Chinese population the risk of ischemic stroke(IS).Methods Using the data from a prospective cohort study of“135”(2013–2019),the polymorphisms of rs2638360 were genotyped in 1,569 unrelated Chinese adults.The SNPassoc package of Rlanguage 3.6.2 was used to evaluate the correlation between the rs2638360 mutation and the risk of IS,and SAS 9.4 was used to perform stratified analysis and crossover analysis by using the Cox proportional hazard model.Results During the 6-year follow-up,68 subjects(4.33%)developed IS.The polymorphisms of rs2638360 in AGTR1 did not show any effect on the risk of IS.Compared with the subjects who carried TT genotype of rs2638360,increased risk of IS was observed in the subjects who carried TC/CC genotype with age≥51 years,or with low-density lipoprotein cholesterol(LDL-C)<2.58mmol/L.Moreover,the rs2638360 polymorphisms combined with age,or with HDL-C might have obvious effects on the risk of IS.Conclusion The subjects who carry rs2638360 TC/CC genotype with age≥51 years,or with low LDL-C levels may be more easy to suffer from IS.Moreover there is a joint effect between rs2638360 polymorphisms and age,or and HDL-C on the risk of IS in Chinese population.