微生物菌剂对连作蒙古黄芪生物量及土壤真菌群落结构的影响

Effects of microbial inoculants on biomass of continuous cropping Astragalus membranaceus var.mongholicus and soil fungal community structure

【目的】明确不同微生物菌剂对连作蒙古黄芪生物量和土壤真菌群落结构的影响。【方法】以蒙古黄芪(Astragalus membranaceus var.mongholicus)为试验材料,设ETS复合菌剂(ETS)、多维特抗菌剂(DWTK)、多功能维生素菌剂(WSS)、空白对照(CK)4个处理,测定蒙古黄芪生物量,并采用高通量测序技术分析土壤真菌群落结构。【结果】DWTK处理对蒙古黄芪地上部和根部生物量有显著促进作用(P<0.05),其中,地上部生物量较CK提高17.5%、根部生物量较CK提高18.4%。3种微生物菌剂对土壤真菌区系的改善效果明显,其中,DWTK处理的土壤真菌OTU数量远高于CK和WSS处理,且真菌类群更加丰富;与其他3个处理相比,DWTK处理病原真菌Fusarium丰度显著降低,真菌Gibberella丰度显著提高。【结论】多维特抗菌剂可以有效改善蒙古黄芪根际真菌群落结构,减少病原真菌数量,提高真菌数量;有利于提高连作蒙古黄芪的抗病性,增加蒙古黄芪生物量。

【Objective】To clarify the effects of different microbial inoculants on biomass of continuous cropping A.membranaceus var.mongholicus and soil fungal community structure.【Methods】Four treatments were set up using A.membranaceus var.mongholicus as test material,ETS compound inoculant(ETS),Doveite antibacterial inoculant(DWTK),multifunctional vitamin bacterial inoculant(WSS)and blank control(CK).The biomass of A.membranaceus var.mongholicus was measured.And high-throughput sequencing technology was used to examine the fungal community structure in the soil.【Results】The biomass of A.membranaceus var.mongholicus above ground tissues and roots was significantly(P<0.05)increased by DWTK treatment,with above ground biomass increased by 17.5%and root biomass increased by 18.4%,compared with CK.The three microbial inoculants clearly improved the soil fungal community.Among them,the number of soil fungi OTU in DWTK treatment was substantially higher than that of CK and WSS treatment,and the fungal groups were more abundant.In comparison to the other three treatments,the DWTK treatment significantly reduced the abundance of pathogenic fungi Fusarium and increased the abundance of Gibberella.【Conclusion】Doveite antibacterial inoculant effectively improved the structure of rhizosphere fungi community of A.membranaceus var.mongholicus,reduced the number of pathogenic fungi,and increased the number of fungi.It was advantageous to strengthen the continuous cropping A.membranaceus var.mongholicus disease resistance and boost the biomass.