结直肠癌组织中CDC5L、DDX21及端粒酶的表达与临床意义

Expression and clinical significance of CDC5L,DDX21 and telomerase in colorectal cancer tissues

目的探讨结直肠癌(CRC)中细胞分裂周期因子5(CDC5L)、DExD/H-box解旋酶(DDX21)及端粒酶(hTERT)的表达及临床意义。方法应用R语言分析人类癌症基因组数据库中CRC癌和癌旁组织中CDC5L、DDX21及hTERT的表达。应用免疫组化检测CRC癌和癌旁组织中CDC5L、DDX21及hTERT的蛋白表达。Pearson线性相关分析CRC癌组织中CDC5L、DDX21及hTERT表达的相关性。统计学分析癌组织中CDC5L、DDX21及hTERT表达与临床病理特征之间的关系。Kaplan-Meier生存分析(Log-rank检验)不同CDC5L、DDX21及hTERT表达患者生存预后的差异。结果相较于癌旁组织,CRC癌组织中CDC5L、DDX21及hTERT mRNA的表达明显升高,差异具有统计学意义(P<0.001)。CRC癌组织中CDC5L、DDX21、hTERT表达阳性率明显高于癌旁组织(均P<0.05)。CDC5L与DDX21表达呈显著正相关(r_(s)=0.576,P<0.001);DDX21与hTERT表达呈显著正相关(r_(s)=0.521,P<0.001)。CDC5L与hTERT表达呈显著正相关(r_(s)=0.628,P<0.001)。不同肿瘤分期及淋巴结转移的CRC癌组织中CDC5L、DDX21及hTERT蛋白表达差异具有统计学意义(均P<0.05)。Kaplan-Meier生存曲线(Log-rank检验)分析结果,CDC5L阳性表达组、DDX21阳性表达组、hTERT阳性表达组患者3年总体生存率为分别明显低于CDC5L阴性表达组、DDX21阴性表达组、hTERT阴性表达组(均P<0.05)。结论CRC癌组织中CDC5L、DDX21及hTERT表达上调,CDC5L、DDX21及hTERT的表达与肿瘤分期及淋巴结转移有关,是新的评估CRC预后的肿瘤标志物。

Objective To study the expression and clinical significance of cell division cycle factor 5(CDC5L),DExD/H-box helicase(DDX21)and telomerase(hTERT)in colorectal cancer(CRC).Methods R language was used to analyze the expressions of CDC5L,DDX21 and hTERT in CRC cancer and adjacent tissues from the Human Cancer Genome Database.The protein expressions of CDC5L,DDX21 and hTERT in CRC carcinoma and adjacent tissues were detected by immunohistochemistry.The correlation of CDC5L,DDX21 and hTERT expression in CRC cancer tissues was analyzed by Pearson linear correlation analysis.The relationship between the expression of CDC5L,DDX21 and hTERT in cancer tissues and clinicopathological characteristics was statistically analyzed.Kaplan-Meier survival analysis(Log-rank test)were used to analyze the difference in survival prognosis of patients with different CDC5L,DDX21 and hTERT expression.Results Compared with adjacent tissues,the expressions of CDC5L,DDX21 and hTERT mRNA in CRC cancer tissues were significantly higher,and the difference was statistically significant(P<0.001).The positive rates of CDC5L,DDX21 and hTERT in CRC cancer tissues were significantly higher than those in adjacent tissues(all P<0.05).There was a significant positive correlation between CDC5L and DDX21 expression(r_(s)=0.576,P<0.001).DDX21 and hTERT expression were significantly positively correlated(r_(s)=0.521,P<0.001).There was a significant positive correlation between CDC5L and hTERT expression were significantly positively correlated(r_(s)=0.628,P<0.001).There were significant differences in the expression of CDC5L,DDX21 and hTERT proteins in CRC cancer tissues with different tumor stages and lymph node metastasis(all P<0.05).Kaplan-Meier survival curve(Log-rank test)analysis results showed that the 3-year overall survival rates of patients in the CDC5L positive expression group,DDX21 positive expression group,and hTERT positive expression group were significantly lower than those in the CDC5L negative expression group,DDX21 negative expression group,and hTERT negative expression group,respectively(all P<0.05).Conclusion The expressions of CDC5L,DDX21 and hTERT are up-regulated in CRC cancer tissues.The expressions of CDC5L,DDX21 and hTERT are related to tumor stage and lymph node metastasis,and are new tumor markers for evaluating the prognosis of CRC.