土豆脲酶提取及基于酶诱导碳酸钙沉淀技术对风积沙改良的方法

Extraction of potato urease and improvement of aeolian sand based on EICP Technology

基于酶诱导碳酸钙沉淀(EICP)技术,使用植物脲酶对沙漠风积沙进行改良加固试验。为寻求价格低廉和操作简单的脲酶提取方法,以土豆为原材料,去离子水、甘油水溶液和乙醇水溶液为提取液,通过破碎冷藏及高速离心方法成功地从土豆中分离提取出植物脲酶,分析提取过程中不同提取液对脲酶提取效果的影响,基于EICP技术,利用土豆脲酶液对风积沙进行改良处理。结果显示,在3种提取液中,效果最优的是乙醇溶液,甘油水溶液次之,去离子水提取效果最差。而当固液比为1:6、乙醇溶液浓度为30%时,脲酶活性值最高且提取效果最佳。在风积沙改良试验中,试件表面形成了坚硬的结皮层并具有较高的抗压强度,随着浸泡脲酶次数的增加,无侧限抗压强度逐渐增强。

Based on enzyme induced calcium carbonate precipitation(EICP)technology,plant urease was used to improve and strengthen desert aeolian sand.In order to find a cheap and simple urease extraction method,potato was used as the raw material,deionized water,glycerol aqueous solution and ethanol aqueous solution were used as extraction solution,and the plant urease in potato was separated and extracted by crushing,refrigeration and high-speed centrifugation,so as to study the influence of different extraction solutions on the extraction effect of urease;The results showed that the best of the three extracts was ethanol solution.When the solid-liquid ratio was 1:6 and the concentration of ethanol solution was 30%,the urease activity value was the highest and the extraction effect was the best.In the improvement experiment of aeolian sand,a hard crust is formed on the surface of the specimen and is of high compressive strength,and the unconfined compressive strength increases gradually with increase of cycles of soaking urease.