摘要
以病死牛病变肺脏组织为研究对象,通过革兰氏染色和PCR鉴定确定多杀性巴氏杆菌分离株的血清型,进行分离菌株的小鼠致病性试验和药敏试验。结果表明:该分离菌株为未知型多杀性巴氏杆菌,对硫酸阿米卡星、左氧氟沙星、硫酸庆大霉素、乳酸环丙沙星高度敏感;小鼠多杀性巴氏杆菌最小致死量为1.2×10^(8)CFU/m L。
In this paper,diseased lung tissue of dead cattle was taken as the research object.The serotype of Pasteurella multocida isolated strain was determined by Gram′s stain and PCR identification,and then pathogenicity testand drug sensitivity test of the isolated strain were carried out in mice.The results showed that the isolated strain wasPasteurella multocida of unknown type,which was highly sensitive to amikacin sulfate,levofloxacin,gentamycin sulfateand ciprofloxacin lactate.The minimum lethal dose of Pasteurella multocida in mice was 1.2×10^(8)CFU/m L.
作者
汪阳
马艳荣
仲荣
金映红
李威
薛晶
汪萍
陈古丽
颜成旭
马丽梅
夏俊
WANG Yang;MA Yanrong;ZHONG Rong;JIN Yinghong;LI Wei;XUE Jing;WANG Ping;CHEN Guli;YAN Chengxu;MA Limei;XIA Jun(Xinjiang Agricultural University,Urumqi Xinjiang 830000;Xinjiang Uygur Autonomous Region Animal Health Supervision Institute,Urumqi Xinjiang 830000;Altay Prefecture Animal Husbandry Workstation,Altay Xinjiang 836500;Institute of Veterinary Medicine,Xinjiang Academy of Animal Science(Animal Clinical Medical Research Center,Xinjiang Academy of Animal Science),Urumqi Xinjiang 830000)
出处
《现代农业科技》
2023年第6期176-178,共3页
Modern Agricultural Science and Technology
基金
新疆维吾尔自治区区域协同创新专项(科技援疆计划)(2021E02032)
2022年新疆维吾尔自治区中央引导地方项目(ZYYD2022B16)
新疆维吾尔自治区重大科技专项“新疆畜禽疫病防控体系质量提升工程”(2022273334)
新疆维吾尔自治区天山英才青年科技拔尖人才项目(青年科技创新人才培养)“牛羊幼畜群发病防控关键技术研究”(2022196128)。
关键词
牛
多杀性巴氏杆菌
分离鉴定
防治
cattle
Pasteurella multocida
isolation and identification
control
