基于全基因组鉴定与分析金钗石斛GRAS基因家族

Identification and Analysis of Dendrobium nobile GRAS Gene Family Based on Genome-wide

【目的】探明金钗石斛(Dendrobium nobile)GRAS基因家族的结构与功能,为研究GRAS家族在金钗石斛生长发育过程中的功能提供参考。【方法】采用全基因组鉴定及分析技术对金钗石斛GRAS家族成员进行鉴定,并进行生物信息学和基因表达模式分析。【结果】金钗石斛基因组中有53个GRAS基因(DnGRAS1~DnGRAS53),大部分GRAS基因不含有内含子,仅DnGRAS1、DnGRAS5、DnGRAS9、DnGRAS10、DnGRAS19、DnGRAS376个GRAS基因有内含子,分布在PAT1、LISCL、SHR、SCR 4个亚族当中;金钗石斛GRAS基因不均匀的分布在染色体上,普遍存在于基因密度较高的区域,并未出现染色体内的串联重复现象,其中,仅有16对GRAS基因具有共线性,具有高度同源性;GRAS基因含有赤霉素响应、低温响应、生长素响应、防御与应激响应等与生长发育相关的顺式作用元件。DnGRAS21和DnGRAS39基因在根中表达最高,在茎、叶、花中也有不同程度表达。【结论】GRAS家族基因在根部表达量较高。推测GRAS家族基因可能在根中起重要的调控作用,从而影响金钗石斛根的生长发育。

【Objective】The structure and function of Dendrobium nobile GRAS gene family is explored to provide a reference for studying the function of GRAS family in the growth and development of D.nobile.【Method】Genome-wide identification and analysis techniques are used to identify D.nobileGRAS family members,and the bioinformatics and gene expression pattern are analyzed.【Result】There are 53 GRAS genes(DnGRAS1~DnGRAS53)in D.nobile genome,most of GRAS genes do not contain introns,and only 6 GRAS genes have introns,respectively DnGRAS1,DnGRAS5,DnGRAS9,DnGRAS10,DnGRAS19 and DnGRAS37,distributing 4 subfamilies of PAT1,LISCL,SHR and SCR;D.nobile GRAS gene distributes in chromosome unevenly,and commonly exists in the area with higher gene density,and there is no tandem duplication in chromosome.Among them,only 16 pairs of GARS gene have collinearity,which have high homology.GRAS gene contains GA response,low temperature response,auxin response,defense and stress response and other cis-acting elements related to growth and development.DnGRAS21 and DnGRAS39 genes are highest expressed in roots and also to varying degrees in stems,leaves,and flowers.【Conclusion】The expression level of GRAS family genes is higher in root.It can be speculated that GRAS family genes may play an important regulatory role in roots,thus affecting the growth and development of D.nobile root.