老年喉鳞状细胞癌中PAC-1、CD62P、SLC7A11表达及临床意义

Expression and clinical significance of PAC-1, CD62P and SLC7A11 in senile laryngeal squamous cell carcinoma

目的 分析老年喉鳞状细胞癌患者血小板表面血小板膜糖蛋白Ⅱb/Ⅲa纤维蛋白原受体(PAC-1)、血小板P-选择素(CD62P)、SLC7A11的表达水平及临床意义。方法 收集我院2016年1月至2021年1月收治的老年喉鳞状细胞癌患者87例(喉癌组),另选取本院同期收治声带息肉患者87例(对照组)。收集患者血液及病理检查标本,采用流式细胞术通过单抗免疫荧光标记测定特异性荧光抗体结合血小板阳性率,进行PAC-1、CD62P检测,免疫组化SP法检测SLC7A11表达。分析PAC-1、CD62P、SLC7A11表达与患者预后的关系。结果 喉癌组中PAC-1、CD62P阳性表达率高于对照组,SLC7A11高表达率(28.74%)低于对照组(62.07%),差异具有统计学意义(P<0.05)。87例患者中预后良好69例,预后不良18例,预后不良组中PAC-1、CD62P阳性表达率高于预后良好组,SLC7A11高表达率(94.44%)高于预后良好组(53.62%),差异有统计学意义(P<0.05)。预后不良者临床分期、颈部淋巴结转移占比明显高于对照组(P<0.05),两组在年龄、性别、分化程度上比较无差异(P>0.05)。以临床分期、颈部淋巴结转移、PAC-1、CD62P、SLC7A11作为自变量,进行多因素Cox回归分析结果显示:临床分期、颈部淋巴结转移、PAC-1、CD62P、SLC7A11是影响患者预后的独立因素(P<0.001)。结论 PAC-1、CD62P、SLC7A11在老年喉鳞状细胞癌患者中均呈高表达,且与患者预后情况密切相关,或可作为患者预后评估的参考指标。

Objective To analyze the expression levels of platelet membrane glycoprotein Ⅱb/Ⅲa fibrinogen receptor(PAC-1), platelet P-selectin(CD62P) and SLC7A11 on platelet surface and their clinical significance in elderly patients with laryngeal squamous cell carcinoma. Methods Eighty-seven elderly patients with laryngeal squamous cell carcinoma admitted to our hospital from January 2016 to January 2021(laryngeal cancer group) were collected, and 87 patients with vocal cord polyp admitted to our hospital during the same period(control group) were selected. Blood and pathological specimens were collected from the patients, and the positive rate of specific fluorescent antibody binding platelets was determined by flow cytometry using monoclonal immunofluorescence labeling, PAC-1 and CD62P were detected, and expression of SLC7A11 was detected by immunohistochemical SP method. The relationship between the expressions of PAC-1, CD62P and SLC7A11 and the prognosis of patients was analyzed. Results The positive expression rate of PAC-1 and CD62P in laryngeal cancer group was higher than that in control group, and the high expression rate of SLC7A11(62.07%) was lower than that in control group(28.74%), the difference was statistically significant(P<0.05). Among the 87 patients with good prognosis, 69 patients had good prognosis, and 18 patients had poor prognosis. The positive expression rate of PAC-1 and CD62P in the poor prognosis group was higher than that in the good prognosis group, and the high expression rate of SLC7A11(94.44%) was higher than that in the good prognosis group(53.62%)(P<0.05). The proportion of clinical stage and cervical lymph node metastasis in patients with poor prognosis was significantly higher than that in the control group(P<0.05), but there were no differences in age, gender and degree of differentiation between the two groups(P>0.05). Multivariate Logistic regression analysis with clinical stage, cervical lymph node metastasis, PAC-1, CD62P and SLC7A11 as independent variables showed that clinical stage, cervical lymph node metastasis, PAC-1, CD62P and SLC7A11 were independent risk for recurrence(P<0.001). Conclusion PAC-1, CD62P and SLC7A11 are highly expressed in elderly patients with laryngeal squamous cell carcinoma, and are closely related to the prognosis of patients, which may be used as a reference indicator for prognosis evaluation.