基于TCGA数据库分析CANX在急性髓系白血病中的表达及其临床意义

Expression and clinical significance of CANX in acute myeloid leukemia based on TCGA database

目的:探索CANX与急性髓系白血病(AML)患者预后及免疫细胞浸润的关系。方法:通过TIMER数据库探索CANX在TCGA肿瘤及正常组织样本中的表达情况。通过R语言发现AML及健康样本间CANX的表达差异。通过Kaplan-Meier法和Log-Rank检验评估CANX表达与AML患者生存的关系。使用R语言分析CANX表达与患者临床性状、药物敏感性及免疫细胞浸润的相关性。结果:CANX在13种肿瘤及正常组织样本间的差异表达具有统计学意义(P<0.05)。CANX高表达与AML患者预后良好相关(P<0.05)。这在GSE37642数据集中得到验证。CANX表达与患者年龄、生存状态、FAB分期及核型均相关(P<0.05)。CANX高表达、低龄及良好染色体核型均是AML预后良好的独立预测因素(P<0.05)。CANX表达可能影响AML患者对多种药物的敏感性(P<0.05)。且CANX表达与M1巨噬细胞及CD8 T细胞表达正相关。结论:CANX可能作为一种新的潜在生物标志物,未来用于预测患者预后并指导患者精准诊疗。

Objective:To explore relationships between CANX expression,prognosis and immune infiltration of acute myeloid leukemia(AML)patients.Methods:The TIMER database was used to compare the CANX expression among a variety of TCGA tumor and normal tissue samples.The difference of CANX expression between AML and normal samples was found by R software.The Kaplan-Meier method and Log-Rank test were used to assess the relationship between CANX expression and patient survival.The R software was used to find correlations between CANX expression,clinical characteristics,drug sensitivity,and immune infiltration in AML.Results:The differential expression of CANX in 13 tumor and normal tissue samples were statistically significant(P<0.05).The high CANX expression was associated with a favorable prognosis(P<0.05),which was validated in GSE37642.The expression of CANX was correlated with age,survival status,FAB stage,and karyotype(P<0.05).High CANX expression,low age and favorable karyotype were independent predictors of a favorable prognosis(P<0.05).CANX expression may affect the sensitivity of AML patients to multiple drugs(P<0.05).The expression of CANX was positively correlated with that of M1 macrophages and CD8 T cells.Conclusion:CANX may be used as a novel potential biomarker,and could benefit AML patients by predicting patient prognosis and providing precise treatment indications.