长链非编码RNA CUST-49525和CUST-40243在1,4-苯醌致K562细胞毒性中的表达

Expression of long noncoding RNA CUST-49525 and CUST-40243 in K562 cytotoxicity induced by 1,4-benzoquinone

目的通过检测LncRNA CUST-49525和CUST-40243在1,4-苯醌致细胞毒性过程中表达的变化,为后期研究两者在细胞毒性中参与的调控作用提供依据。方法使用0、10、20、40μmol/L的1,4-苯醌染毒K562细胞24 h,光学显微镜下观察细胞形态特征;采用CCK-8法测定细胞生长情况;采用流式细胞术分析细胞周期分布和凋亡情况;使用实时荧光定量PCR法测定LncRNA CUST-49525和CUST-40243表达情况。结果1,4-苯醌染毒K562细胞24 h后镜下观察细胞数量和形态发生明显改变,与对照组相比,20和40μmol/L剂量组K562细胞相对增殖率显著降低(P<0.01);各剂量组1,4-苯醌均可诱导细胞发生凋亡,呈现浓度依赖性(P<0.01),与对照组相比,20和40μmol/L剂量组K562细胞G1期比例增加(P<0.01),S期比例下降(P<0.05);实时荧光定量PCR结果显示,与对照组相比,20和40μmol/L剂量组K562细胞中LncRNA CUST-49525和CUST-40243表达量增加(P<0.01)。结论LncRNA CUST-49525和CUST-40243在1,4-苯醌致K562细胞毒性过程中异常表达,两者可能参与调控细胞毒性过程,但其具体作用机制还需要进一步研究。

Objective To detect the expression changes of LncRNA CUST-49525 and CUST-40243 in the process of cytotoxicity induced by 1,4-benzoquinone,provide the basis for the later study of their regulatory role in cytotoxicity.Methods K562 cells were exposed to 0,10,20 and 40μmol/L 1,4-benzoquinone for 24 hours,and the morphological characteristics of the cells were observed under optical microscope.CCK-8 method was used to measure the cell growth.Cell cycle distribution and apoptosis were analyzed by flow cytometry.The expression of LncRNA CUST-49525 and CUST-40243 was measured by real-time fluorescence quantitative PCR.Results After K562 cells were exposed to 1,4-benzoquinone for 24 hours,the number and morphology of K562 cells were obviously changed under microscope.Compared with the control group,the relative proliferation rate of K562 cells in20 and 40μmol/L dosage groups decreased significantly(P<0.01).1,4-benzoquinone in each dose group could induce cell apoptosis in a concentration-dependent manner(P<0.01).Compared with the control group,the G1 phase proportion of K562 cells in the20 and 40μmol/L dosage groups increased(P<0.01),while the S phase proportion decreased(P<0.05).The results of real-time quantitative PCR showed that compared with the control group,the expression levels of LncRNA CUST-49525 and CUST-40243in K562 cells in 20 and 40μmol/L dosage groups increased(P<0.01).Conclusion LncRNA CUST-49525 and CUST-40243 are abnormally expressed in the cytotoxicity of K562 cells induced by 1,4-benzoquinone,and they may be involved in the regulation of cytotoxicity,but their specific mechanism needs further study.