藿乌粉的提取工艺研究

Study on Extraction Technology of Huowu Power

为了优化藿乌粉的提取工艺,研究以乙醇浓度、固液比、提取时间、提取次数为影响因素,以2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷、淫羊藿苷、朝藿定A、朝藿定B含量以及干膏得率为评价指标,采用层次分析法(AHP)、基于指标相关性的权重确定方法(CRITIC)、AHP-CRITIC混合加权法确定各评价指标的权重系数,根据单因素和正交试验结果优化提取工艺参数。结果表明AHP-CRITIC混合加权法确定出的权重系数更为科学、合理,优选出的最佳提取工艺为12倍量60%乙醇提取2次,每次1.5 h。3次验证试验综合评分均值为89.69,RSD值为0.63%。优选出的藿乌粉提取工艺经验证稳定可行,重复性好,可用于藿乌粉的制备。

In order to optimize the extraction process of Huowu powder.The extraction parameters of Huowu power were optimized according to the single factor test and orthogonal test where the ethanol concentration,solid-liquid ratio,extraction time,and number of extractions were used as influencing factors.The total amounts of 2,3,5,4′-tetrahydrostilbene-2-O-β-D-glucoside,Icariin,Epimedin A,Epimedin B and the yield of dry paste were used as comprehensive evaluation indicators and their respective weighting coefficients were determined by analytic hierarchy process(AHP),criteria importance through intercriteria correlation(CRITIC)method and mixed weighted AHP-CRITIC.The results showed that the weight coefficient determined by AHP-CRITIC is more scientific and reasonable,and the best extraction process is 12 times the amount of 60%ethanol for two times,and each turn for 1.5 h.The mean comprehensive score of the three validation tests was 89.69,and the RSD was 0.63%.The optimized extraction parameters of Huowu power have been verified to be stable,feasible and reproducible for the production.