miR-124、NSE、S100B在新生儿缺氧缺血性脑病中的表达及意义

Expression and significance of miR-124,NSE and S100B in neonatal hypoxic-ischemic encephalopathy

目的 检测新生儿缺氧缺血性脑病(HIE)中枢神经特异性蛋白(S100B)、神经元特异性烯醇化酶(NSE)和miR-124的表达水平,并探究其辅助诊断的价值。方法 选取2020年12月—2021年12月蚌埠医学院第一附属医院HIE患儿50例(HIE组),同期出生的健康新生儿50例(健康组),均于生后2 h内采集脐带血,采用实时荧光定量(qRT-PCR)法检测miR-124水平,并于生后6 h内采集新生儿股静脉血,采用ELISA方法检测S100B、NSE表达水平,建立受试者操作特征(ROC)曲线,评估上述生物标志物在HIE中的临床意义。结果 与健康新生儿比较,HIE患儿的miR-124表达水平降低(0.63±0.11 vs.0.90±0.22),S100B和NSE水平显著增高[0.45(0.34,0.78)μg/L vs.0.33(0.24,0.50)μg/L,39.30(23.71,67.48)μg/L vs.21.16(16.27,27.25)μg/L],差异均有统计学意义(均P<0.05),且不同程度HIE患儿miR-124、S100B、NSE比较差异均有统计学意义(均P<0.05)。ROC分析结果显示血清miR-124、S100B、NSE诊断HIE的曲线下面积AUC分别为0.897、0.690、0.801,miR-124、S100B和NSE联合检测诊断HIE的AUC为0.908,诊断效能最佳。HIE新生儿行为神经评估(NBNA)评分与S100B、NSE呈负相关关系,与miR-124呈正相关关系。结论 HIE新生儿脐带血中miR-124表达水平降低,miR-124联合S100B及NSE对HIE的诊断价值较高。

Objective To investigate the expression levels of central neuron-specific protein(S100B),neuron-specific enolase(NSE) and miR-124 in neonatal hypoxic-ischemic encephalopathy(HIE) and to explore their diagnostic value.Methods A total of 100 full-term neonates in the First Affiliated Hospital of Bengbu Medical College from December 2020 to December 2021 were selected,including 50 neonates in the HIE group and 50 neonates in the healthy group.Umbilical cord blood was collected within 2 hours after birth,and the level of miR-124 was detected by real-time fluorescence quantification PCR(qRT-PCR);and femoral vein blood was collected from neonates within 6 hours after birth to detect S100B and NSE levels by ELASA,and the receiver operating characteristic(ROC) curve was constructed to evaluate the clinical significance of the above biomarkers in HIE.Results Compared to healthy neonates,the expression level of miR-124 was decreased in neonates with HIE(0.63±0.11 vs.0.90±0.22),whereas the levels of S100B and NSE were significantly increased [0.45(0.34,0.78) μg/L vs.0.33(0.24,0.50) μg/L,39.30(23.71,67.48) μg/L vs.21.16(16.27,27.25) μg/L],the differences were statistically significant(all P<0.05),and there were statistically significant differences in miR-124,S100B and NSE in neonates with different HIE levels(all P<0.05).ROC analysis results showed that the area under the curve AUCs of serum miR-124,S100B and NSE for HIE diagnosis were 0.897,0.690 and 0.801,respectively,and the AUC of combined detection of miR-124,S100B and NSE for HIE diagnosis was 0.908,indicating the best diagnostic efficiency.The Neonatal Behavioral Neurological Assessment(NBNA) score of HIE was negatively correlated with S100B and NSE,and positively correlated with miR-124.Conclusion The expression level of miR-124 in umbilical cord blood of neonates with HIE is decreased,and miR-124 combined with S100B and NSE is of high diagnostic value for HIE.