摘要
醌氧化还原酶的检测对提高癌症的诊断效果和预测药物的使用情况都十分重要,通过6步反应成功合成了一种用于醌氧化还原酶检测的荧光探针DBD-Q-1,探针由识别基团三甲基对苯醌和荧光团组成,使用核磁共振和高分辨质谱进行表征确定了其结构.随着醌氧化还原酶浓度的增大,荧光探针的荧光逐渐增强.在此过程中荧光探针DBD-Q-1结构中的三甲基对苯醌部分脱去形成内酯结构,从而导致探针荧光增强.当向其中加入其他生命相关物质时,荧光探针的荧光并没有明显改变,探针表现出对醌氧化还原酶良好的选择性.荧光探针DBD-Q-1在细胞中展现出了对醌氧化还原酶良好的成像效果.
The detection of quinone oxidoreductase 1 is very important to improve the diagnostic effect of cancer and predict the use of drugs. A fluorescent probe DBD-Q-1 for the detection of quinone oxidoreductase 1 is successfully synthesized through a six-step reaction. The probe consists of the recognition group trimethyl p-benzoquinone and a fluorophore, and its structure is characterized by nuclear magnetic resonance and high-resolution mass spectrometry to confirm. With the increase of the concentration of quinone oxidoreductase 1, the fluorescence of the fluorescent probe gradually increases. In the process, the trimethyl p-benzoquinone in the structure of the fluorescent probe DBD-Q-1 is removed to form lactone, resulting in enhanced fluorescence of probe. When other life-related substances are added, the fluorescence of the probe does not change significantly, and the probe shows good selectivity for quinone oxidoreductase 1. The fluorescent probe DBD-Q-1 shows good cell imaging for quinone oxidoreductase 1.
作者
高旭
焦扬
段春迎
GAO Xu;JIAO Yang;DUAN Chunying(Zhang Dayu School of Chemistry,Dalian University of Technology,Dalian 116024,China;School of Chemical Engineering,Dalian University of Technology,Dalian 116024,China)
出处
《大连理工大学学报》
CAS
CSCD
北大核心
2023年第2期119-124,共6页
Journal of Dalian University of Technology
基金
中央高校基本科研业务费专项资金资助项目(DUT20LK12)。
关键词
醌氧化还原酶
荧光探针
制备
性能
quinone oxidoreductase 1
fluorescent probe
preparation
properties