秋季红香椿芽冷藏期间花青素含量变化及基因表达分析

Anthocyanins Contents Changes and Genes Expressions Analysis of Autumn Red Toona sinensis Buds during Cold Storage

以秋季红香椿芽为试材,测定其冷藏期间的花青素和叶绿素含量,同时利用转录组数据对红香椿芽中花青素合成基因进行鉴定,并利用实时荧光定量PCR对红香椿芽花青素合成相关基因的相对表达量进行分析,旨在研究秋季红香椿芽在冷藏期间花青素的变化机理。结果表明:利用转录组共鉴定出9个与红香椿芽花青素相关的基因(TsPAL、TsC4H、TsCHS、TsCHI、TsF3H、TsF35’H’、TsDFR、TsANS和Ts3GT);秋季红香椿芽叶片花青素和叶绿素含量均高于叶柄,且随着冷藏期的延长,红香椿芽花青素含量下降,叶绿素含量在3 d时均升高;除TsPAL和TsC4H基因外,其他与红香椿芽花青素合成相关基因的相对表达量在冷藏期间整体呈下降趋势;相关性分析显示,叶柄中TsPAL、TsC4H和TsCHS基因的相对表达量与花青素含量呈负相关,其余基因的相对表达量与花青素含量呈正相关。

In this paper,the autumn red T.sinensis buds were taken as test materials,and the anthocyanins and chlorophylls contents during refrigeration were measured,the anthocyanin synthesis genes were identified using transcriptome data,and the relative expressions of genes related to anthocyanin synthesis were analyzed using real-time fluorescence quantitative PCR,aiming to study the change mechanisms of anthocyanins in red T.sinensis buds during refrigeration.The results showed that 9 anthocyanin-related genes(TsPAL,TsC4H,TsCHS,TsCHI,TsF3H,TsF35’H’,TsDFR,TsANS and Ts3GT) were identified by transcriptome.In autumn,the contents of anthocyanins and chlorophylls in leaves were higher than those in petioles.With the extension of cold storage period,the contents of anthocyanins in leaves were degraded,and the contents of chlorophylls were increased at 3 days.Except for TsPAL and TsC4H genes,the relative expression levels of other anthocyanins genes in red T.sinensis buds showed an overall downward trend during cold storage.Correlation analysis showed that the relative expression levels of TsPAL,TsC4H and TsCHS genes in petioles were negatively correlated with anthocyanins contents,while the relative expression levels of the remaining genes were positively correlated with anthocyanins contents.