铁碳纤维人工水草对污染河湖微生物挂膜的影响

Effects of iron and carbon fiber artificial aquatic mat on microbial membrane hanging in polluted rivers and lakes

针对水体强化净化过程中碳纤维人工水草表面能低、微生物附着时间长、挂膜启动慢的问题,利用废铁和碳纤维水草组合构建了铁碳纤维人工水草(ICF),以重污染地表水为原水,在阶段曝气的条件下进行了强化挂膜实验研究。结果表明,与碳纤维人工水草相比,铁碳纤维人工水草上生物膜的附着性能显著提升,生物膜的挂膜率由125.1%增加至133.0%,形成生物膜的团簇结构尺寸明显更大。启动期末(21 d)生物膜胞外聚合物中多糖、蛋白及蛋白/多糖比,分别由53,71 mg/gVSS、1.33改变为39,106 mg/gVSS、2.71。铁碳材料对假单胞菌属细菌的富集和筛选,改善了细菌的运动能力,促进了细菌分泌胞外聚合物的行为,此外,微生物活性功能基因在ICF中高度表达,提升了铁碳纤维材料表面微生物附着性能。

In order to solve the problems of low surface energy,long microbial attachment time and slow start of membrane hanging in the process of enhanced water purification,an iron carbon fiber-CF artificial water mat was constructed by combining waste iron and carbon fiber water mat.The heavily polluted surface water was used as raw water.Under the condition of stage aeration,the experiment of enhanced membrane hanging was carried out.The results showed that compared with carbon fiber artificial aquatic mat,the adhesion performance of biofilm on iron carbon fiber artificial aquatic mat was significantly improved,the hanging rate of biofilm increased from 125.1%to 133.0%,and the size of biofilm cluster structure was significantly larger.The polysaccharide,protein and protein-to-polysaccharide ratio in the extracellular polymer of biofilm changed from 53,71 mg/gVSS and 1.33 to 39,106 mg/gVSS and 2.71 at the end of the initiation period(21 d),respectively.The enrichment and screening of Pseudomonas bacteria by iron-carbon materials improved the movement ability of bacteria and promoted the secretion of extracellular polymers by bacteria.In addition,the highly expressed functional genes of microorganisms were highly expressed in ICF,which improved the adhesion performance of microorganisms on the surface of iron-carbon fiber materials.