外周血CD3^(+)T细胞上程序性死亡因子-1 T细胞免疫球蛋白及免疫受体酪氨酸抑制基序在子宫内膜异位症中的表达和价值

Abnormal expression of programmed cell death protein 1 and T-cell immunoglobulin and immunoreceptor tyrosine inhibitory motif on CD3^(+)T cells in peripheral blood of patients with endometriosis

目的探讨外周血CD3^(+)T细胞上程序性死亡因子-1(PD-1)、T细胞免疫球蛋白及免疫受体酪氨酸抑制基序(TIGIT)在子宫内膜异位症(EM)的表达和价值。方法采用前瞻性病例-对照研究,收集在复旦大学附属妇产科医院诊断为EM的患者(EM组)和相同年龄的子宫肌瘤者(对照组)的外周血。采用流式细胞术检测抗凝外周血中CD3^(+)T细胞上表面分子PD-1和TIGIT的百分含量,并比较在两组间的差异。结果两组患者的年龄、体质指数(BMI)及产次比较差异均无统计学意义(均P>0.05),子宫内膜异位症组研究对象有痛经症状的比例显著高于对照组(P<0.001)。EM患者外周血中CD3^(+)T细胞占淋巴细胞的比例为(65.73±12.10)%,与对照组组中CD3^(+)T细胞(64.98±7.22)%比较,差异无统计学意义(P>0.05)。PD-1^(+)CD3^(+)T细胞占CD3^(+)T细胞的比例在EM组中为(25.57±8.01)%,显著高于对照组中的(21.46±7.15)%,差异有统计学意义(P<0.05)。TIGIT^(+)CD3^(+)T细胞占CD3^(+)T细胞的比例在EM组中为(10.23±2.80)%,显著高于对照组中的(8.90±2.13)%,差异有统计学意义(P<0.05)。结论PD-1和TIGT在EM患者外周CD3^(+)T细胞中表达异常升高,提示其与EM的发生可能相关。

Objective To investigate the expression and value of programmed cell death protein 1(PD-1)and T cell immunoglobulin and immunoreceptor tyrosine inhibitory motif(TIGIT)on peripheral blood CD3^(+)T cells in patients with endometriosis(EM).Methods A prospective case-control study was conducted.Peripheral blood samples were collected from patients diagnosed with EM(EM group)and uterine fibroids of the same age(control group)in Obstetrics and Gynecology Hospital of Fudan University.The percentages of PD-1 and TIGIT on the CD3^(+)T cells in anticoagulated peripheral blood were detected by flow cytometry,and the differences between the two groups were compared.Results There was no significant difference in age,body mass index(BMI)and parity between the two groups(P>0.05).The proportion of subjects with dysmenorrhea in the EM group was significantly higher than that in the control group(P<0.001).The proportion of CD3^(+)T cells in peripheral blood of EM patients was(65.73±12.10)%,which was not significantly different from that of control group(64.98±7.22)%(P>0.05).The proportion of PD-1^(+)CD3^(+)T cells in the EM group was(25.57±8.01)%,which was significantly higher than that in the control group(21.46±7.15)%,and the difference was statistically significant(P<0.05).The proportion of TIGIT^(+)CD3^(+)T cells in the EM group was(10.23±2.80)%,which was significantly higher than that in the control group(8.90±2.13)%,and the difference was statistically significant(P<0.05).Conclusion The expression of PD-1 and TIGT in peripheral CD3^(+)T cells of patients with EM is abnormally elevated,suggesting that PD-1 and TIGT may be related to the occurrence of EM.