基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱技术的藏药八味小檗皮胶囊化学成分分析研究

Chemical Composition Analysis of Tibetan Medicine Bawei Xiaobopi Capsules Based on UPLC-Q-Exacutive Orbitrap MS Technology

目的:采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Exacutive Orbitrap MS)技术对八味小檗皮胶囊的化学成分进行分析鉴定。方法:采用ZORBAX SB-C18柱进行色谱分离,以甲醇-0.1%甲酸溶液为流动相,采用HESI离子源,在正负离子模式下,捕捉化合物的精准相对分子质量及二级碎片离子信息,结合ChemSpider、mzCloud和mzVault数据库,并查阅相关文献,对八味小檗皮胶囊中所含的化学成分进行快速、准确的定性分析。结果:共鉴定出70种化学成分,包括12种生物碱类、19种黄酮类、6种胆汁酸类、19种有机酸类、3种三萜皂苷类、3种香豆素类和8种氨基酸类成分。结论:本研究首次对八味小檗皮胶囊的化学成分进行了全面的定性表征,为其进一步的质量控制和药效物质基础奠定了基础。

Objective:UPLC-Q-Exacutive Orbitrap MS technology was used to analyze and identify the chemical components of Bawei Xiaobopi capsules.Methods:A ZORBAX SB-C18column was used for chromatographic separation.Methanol-0.1%formic acid solution was used as mobile phase.HESI ion source was used to capture the precise relative molecular weight and secondary fragment ion information of the compounds in positive and negative ion mode.This study referred to ChemSpider,mzCloud and mzVault databases,and consulted relevant literatures to conduct a fast and accurate qualitative analysis of the chemical components in Bawei Xiaobopi capsules.Results:A total of 70 chemical constituents were identified,including 12 alkaloids,19 flavonoids,6 bile acids,19 organic acids,3 triterpenoid saponins,3 coumarins and 8 amino acids.Conclusion:This study was the first to comprehensively characterize the chemical constituents of Bawei Xiaobopi capsules,which laid the foundation for its further quality control and research on effective substances.