基于姜黄素负载的上转换纳米传感体系测定生物样品中的铜离子

Curcumin-loaded upconversion nanosensor for the detection of copper ion in biological samples

通过高温共沉淀法制备了发光效率高、形貌规则、粒径均一的上转换纳米粒子;采用反相微乳法合成二氧化硅壳层(SiO_(2)),实现上转换纳米粒子从油相到水相的转移;将介孔二氧化硅壳层(mSiO_(2))包覆在上转换纳米粒子表面,荧光响应分子姜黄素被负载在m SiO_(2)的孔道中。基于荧光共振能量转移的原理,构建“Turn on”纳米传感体系,并用于Cu^(2+)的检测,检测线性范围为10~50μmol/L,检出限为0.5μmol/L。本方法可实现大鼠血清样品中Cu^(2+)的检测。

An upconversion nanoparticle with high fluorescence efficiency,regular morphology and narrow size distribution was prepared by high temperature co-precipitation method.The silica shell coated on the surface of upconversion nanoparticle was synthesized by the inverse microemulsion method,transferring upconversion nanoparticle from the oil phase to the water phase.A layer of mesoporous silica(mSiO_(2))shell was further synthesized to load curcumin for detection of Cu^(2+).Due to the overlap of the absorption of curcumin and the emission of upconversion nanoparticle,the upconversion luminescence(UCL)was quenched based on fluorescence resonance energy transfer(FRET).In the presence of Cu^(2+),the absorbance of curcumin decreased sharply,resulting in the recovery of UCL.The recoveries of UCL showed a good linear relationship with Cu^(2+)concentration from 10 to 50μmol/L and the detection limit was 0.5μmol/L.The developed nanosensor can be used for detection of the Cu^(2+)in rat serum samples.