伴侣蛋白辅助工业酿酒酵母实现碳中和应用

Chaperone Assisted Industrial Saccharomyces Cerevisiae for Carbon Neutralization

为提高工业酿酒酵母生产燃料乙醇的产量,将卡尔文循环中的Rubisco酶基因转入了工业酿酒酵母,构建了异源固碳通路并进行了转录水平、表达水平和发酵水平的验证。结果显示:未经密码子优化的固碳基因转入工业酿酒酵母后,其密码子适应性较低,不能正确转录相应RNA,但密码子优化后的固碳通路在groE伴侣蛋白的辅助下可以正常表达且糖醇转化率有所提高。研究结果提示工业酿酒酵母生产燃料乙醇是具有可行性的,但需要在工业培养条件下继续菌株优化和适应性进化。

In order to improve the production of fuel ethanol by Industrial Saccharomyces cerevisiae,the Rubisco gene from Calvin-cycle was transferred into industrial Saccharomyces cerevisiae,and the heterologous carbon-fixation-pathway was constructed.The results showed that the codon adaptation of carbon fixation gene without codon optimization was low,and the corresponding RNA could not be correctly transcribed.However,the carbon-fixation-pathway with codon optimization could be normally expressed with the help of groE chaperone,and the sugar-alcohol conversion rate was improved.The results suggest that it is feasible for industrial Saccharomyces cerevisiae to produce fuel ethanol,but it is necessary to continue the optimization and adaptive evolution of fermentation under industrial culture conditions.