响应面试验优化日本冷杉针叶精油提取工艺

Optimization for Extraction of Essential Oil from Needles of Abies firma by Response Surface Methodology

利用响应面(RSM)分析优化日本冷杉Abies firma针叶精油的提取工艺。以日本冷杉精油得率为考察指标,在样品粉碎粒度、浸泡时间、蒸馏时间、蒸馏速度4个单因素实验基础上,通过响应面法的Box-Behnken实验设计对日本冷杉针叶精油水蒸气蒸馏法提取工艺进行优化,建立二次多项式回归方程的预测模型。日本冷杉针叶精油的优化提取工艺为:粉碎粒度200目、浸泡时间37 min、蒸馏时间108 min、蒸馏速度2.8 mL·min^(-1)。在此优化条件下的精油最大得率为0.714%,与预测值0.716%具有很好的一致性,表明采用响应面优化得到的日本冷杉精油提取工艺条件可靠,可为日本冷杉针叶中精油的提取方法提供参考。

In September 2020,needles of introduced 20-year Abies firma were collected from Jinning Caoyutang Forest Park,Zhejiang province.Essential oil from needles were prepared by steam distillation with water as solvent,and response surface methodology(RSM)was used to optimize the extraction process.Taking the yield of essential oil as objective,four single factors were tested like particle size,soaking time,distillation time and distillation speed.The extraction process of A.firma essential oil by steam distillation was optimized through Box Behnken design of response surface methodology,and a prediction model of quadratic polynomial regression equation was established.The optimal extraction process of the essential oil from A.firma needles was as follows:200 mesh of particle size,37 minutes of soaking,108 minutes of distillation,and 2.8 mL/min of the distillation speed.Under this condition,the maximum yield of essential oil was 0.714%,which was in good conformity with the predicted value of 0.716%,indicating that the extraction process was reliable.