LncRNA CRNDE在喀什地区维吾尔族结直肠癌患者中的表达及临床意义

Expression of long non-coding RNA CRNDE and its clinical significance in Uyghur patients with colorectal carcinoma in Kashgar

目的 探讨LncRNA CRNDE在喀什地区维吾尔族结直肠癌患者中的表达及其临床意义。方法 收集喀什地区第一人民医院2016-01—2020-12结直肠癌组织206例,采用原位杂交技术检测石蜡组织标本中LncRNA CRNDE的表达,分析其表达的临床意义。结果 在喀什地区维吾尔族结直肠癌患者中,LncRNA CRNDE在结直肠癌组织标本中的表达高于正常结直肠黏膜组织,其表达水平在结直肠癌的临床病理参数如不同分化、TNM分期、浸润深度、淋巴结转移及远处转移中差异有统计学意义(P均<0.05),而在患者的不同性别、年龄、肿瘤大小及位置中的表达差异无统计学意义(P均>0.05)。另比较LncRNA CRNDE在广东省和新疆喀什地区结直肠癌患者中的表达,发现仅在肿瘤高分化病例中存在差异(P<0.05),在中、低分化及TNM分期中均无明显差异(P均>0.05)。结论 LncRNA CRNDE在喀什地区维吾尔族结直肠癌组织标本高表达,在肿瘤不同分化、TNM分期、浸润深度、是否有淋巴结转移及远处转移中的表达水平有明显统计学差异。在广东省汉族和喀什地区维吾尔族结直肠癌患者高分化病例间,LncRNA CRNDE的表达存在显著差异,而其在不同TNM分期、中低分化病例间不存在民族、地域差异。

Objective To investigate the expression of LncRNA CRNDE in Uyghur patients with colorectal cancer in Kashgar, and to analyse the clinical significance. Methods This retrospective study investigated a cohort of 206 formalin-fixed and paraffin-embedded(FFPE) tissue samples of colorectal carcinoma, diagnosed from January 2016 to December 2020 at the First People’s Hospital of Kashi Prefecture. In situ hybridization was used to detect the expression of LncRNA CRNDE, and the relationship between its expression and clinicopathological characteristics was analyzed. Results Among Uyghur colorectal cancer patients in Kashgar, the expression of LncRNA CRNDE in cancer tissues was obviously higher than that of normal mucosa tissues. Simultaneously, there were statistically significant differences of its expression in the degree of differentiation, TNM stage, depth of invasion, lymph node metastasis and distant metastasis(P<0.05), while there were no significant differences in the gender, age, tumor size and tumor site(P>0.05). In addition, notable difference was only observed in well differentiation group(P<0.05), but not found in medium and poor differentiation group and TNM stage(P>0.05), as compared with the expression of LncRNA CRNDE of patients in Guangdong province. Conclusion LncRNA CRNDE is highly expressed in colorectal cancer tissues of Uygur patients in Kashgar. Meanwhile, there are significant differences of its expression in the degree of differentiation, TNM stage, depth of invasion, lymph node metastasis and distant metastasis. Concurrently, in Guangdong province and Kashgar Region, the expression of LncRNA CRNDE has no ethnic or regional difference in the clinicopathological parameters such as TNM stage, medium and poor differentiation group, except for the well-differentiated group.