血清维生素D与男性精液参数及性激素水平的相关性

Correlation of serum vitamin D with semen parameters and sex hormone levels in men

目的探索血清维生素D水平与男性精液参数、血清性激素之间的相关性。方法本研究不育组为2020年10月—2021年10月期间因不育症于我院生殖中心接受辅助生殖治疗的56例男性;可生育组为同期就诊的56例既往有生育史的男性。使用计算机辅助精液分析系统评估精子活力和浓度;通过精子形态学染色镜下分析评估精子正常形态率;通过染色质扩散法检测精子DNA碎片指数,评估精子染色质完整性;通过ELISA法检测血清中促黄体生成素、促卵泡激素、睾酮以及25-羟基维生素D[25(OH)D]水平,分析血清25(OH)D水平与精液参数以及性激素之间的相关性。结果可生育组的血清25(OH)D水平明显高于不育组(P=0.002)。血清25(OH)D浓度与精液参数包括精子活力(r=0.483,P<0.001)和精子总数(r=0.216,P=0.019)呈正相关性。此外,血清25(OH)D浓度与睾酮水平呈正相关(r=0.210,P=0.025)。血清25(OH)D浓度与精子形态、促黄体生成素、促卵泡激素水平均无显著相关性。结论血清25(OH)D与精液参数(精子总数、精子活力)和血清睾酮之间有着显著的相关性。血清25(OH)D有可能通过影响精子总数、精子活动能力、血清睾酮水平在男性生殖过程中发挥作用,但具体的作用机制还需进一步研究。

Objective To explore the correlation between serum vitamin D level and male semen parameters and serum sex hormones.Methods A total of 56 men who received assisted reproductive treatment in our hospital during Oct.2020 and Oct.2021 were included in the infertility group,and 56 male patients with previous reproductive histories who were treated due to other diseases during the same period were enrolled as the control group.The sperm motility and concentration were assessed using a computer assisted semen analysis system(CASA).The normal sperm morphology rate was evaluated with sperm morphological staining.Sperm DNA fragmentation index(DFI)was detected with chromatin diffusion method to evaluate sperm chromatin integrity.Serum levels of luteinizing hormone(LH),follicle-stimulating hormone(FSH),testosterone(T)and 25-hydroxyvitamin D[25(OH)D]were detected with ELISA.The correlation between serum^(2)5(OH)D level and semen parameters and sex hormones was analyzed.Results Serum^(2)5(OH)D level was significantly higher in the control group than in the infertile group(P=0.002).It was positively correlated with semen parameters,including sperm motility(r=0.483,P<0.001),sperm count(r=0.216,P=0.019),and testosterone level(r=0.210,P=0.025).There was no significant correlation between serum^(2)5(OH)D concentration and sperm morphology,LH and FSH levels.Conclusion Serum^(2)5(OH)D is significantly correlated with and affects total sperm count,sperm motilityand serum testosterone,thus playing a role in male reproduction.The specific mechanism needs further study.