不同形态硒及用量对幼苗期谷子生长与生理的影响

Effects of Different Forms Selenium and Dosage on Foxtail Millet Growth and Physiology at Seedling Stage

为探究不同形态硒及用量对谷子生长和抗性的影响,选用0.0、0.1、0.5、1.0、10.0mmol/L亚硒酸钠和纳米硒,通过水培试验研究不同形态硒浓度对谷子株高、茎粗、根系、硒含量和酶活性的影响。结果表明,2种形态硒随着浓度的升高,株高均呈先升高后降低的趋势;亚硒酸钠对茎粗的影响呈开口向下的抛物线趋势,纳米硒对茎粗有显著的抑制作用。亚硒酸钠对根长和根冠比的影响呈先增加后降低的趋势;低浓度纳米硒对根长影响不显著,高浓度纳米硒对根长有显著的抑制作用;纳米硒对谷子根冠比抑制作用明显。2种形态硒均可提高谷子的硒含量;2种形态硒及其不同浓度对超氧化物歧化酶、过氧化氢酶活性及蛋白质含量均有显著影响,对过氧化物酶活性影响不显著。结合Circos-弦图、谷子株高、茎粗、根系、硒含量和酶活性等试验结果,得出喷施0.1mmol/L的纳米硒溶液对谷子生长、硒含量及酶活性提高效果显著,适宜在谷子种植中应用。

In order to explore the effects of different forms selenium and dosage on foxtail millet growth and resistance,0.0,0.1,0.5,1.0 and 10.0mmol/L sodium selenite and nano selenium were selected to carry out hydroponic culture experiment on foxtail millet,and to explore the effects of different forms and concentrations of selenium on plant height,stem diameter,root system,selenium content and enzyme activity of foxtail millet.The results showed that,with the increase of selenium concentration,the plant height increased first and then decreased.The effects of sodium selenite on stem diameter showed a parabola trend,and nano selenium had a significant inhibitory effects on stem diameter.The effects of sodium selenite on root length and root shoot ratio increased first and then decreased.Low concentration of nano selenium had no significant effects on root length,while high concentration of nano selenium had a significant inhibitory effects on root length.Nano selenium significantly inhibited the root shoot ratio of foxtail millet.Both forms of selenium could increase the selenium content of foxtail millet.The two forms and concentrations of selenium had significant effects on SOD,CAT activities and protein content,but had no significant effects on POD activity.Combined with the experimental results of Circos-string diagram,plant height,stem diameter,root,selenium content and enzyme activity,it was concluded that spraying 0.1mmol/L nano-selenium solution significantly improved foxtail millet growth,selenium content and enzyme activity,and was suitable for the application in foxtail millet planting.