烟苗生长发育对不同氮源形态的响应

Effects of Different Nitrogen Sources on the Growth and Development of Tobacco Seedlings

为探讨不同形态氮源对烟苗素质和生长发育的影响,以烤烟新品系HN2146为参试材料,采用水培试验研究硝酸铵、硝酸钙、硫酸铵和尿素4种不同形态氮源对烟苗生长发育特性的影响。结果表明,烟苗最大叶面积以硝酸钙处理最大,为100.4cm^(2);烟苗叶和根系蛋白质含量以硫酸铵处理最高,分别为151.452和79.367μg/g FW;硝酸还原酶活性表现为硝酸钙>硝酸铵>硫酸铵>尿素处理;烟苗对氮的吸收能力表现为硝酸铵>硫酸铵>硝酸钙>尿素处理。通过对不同处理烟苗相关农艺性状和生理特性指标的主成分分析,其综合得分表现为硝酸钙>硝酸铵>硫酸铵>尿素处理。可见以硝态氮和铵态氮作为唯一氮源对烟苗生长发育的效果较好,而以尿素作为唯一氮源的效果最差,本研究结果为不同氮源在烤烟育苗培育上的应用提供了理论依据。

In order to explore the effects of different nitrogen forms sources on the quality,growth and development of tobacco seedlings,a new flue-cured tobacco strain HN2146 was used as material.The effects of four different nitrogen forms on the growth and development characteristics of tobacco seedlings were carried out by hydroponic experiments with ammonium nitrate(NH_(4)NO_(3)),calcium nitrate[Ca(NO_(3))_(2)],ammonium sulfate[(NH_(4))_(2)SO_(4))]and urea[CO(NH_(2))_(2)].The results showed that the maximum leaf area of tobacco seedling was 100.4cm^(2) with the calcium nitrate treatment.The highest protein content in tobacco leaves and roots were the treatment with ammonium sulfate,which were 151.452 and 79.367μg/g FW,respectively.The activity of nitrate reductase shown calcium nitrate>ammonium nitrate>ammonium sulfate>urea treatment.The absorption capacity of nitrogen shown ammonium nitrate>ammonium sulfate>calcium nitrate>urea treatment.Through the principal component analysis of the relevant agronomic traits and physiological characteristics of tobacco seedlings in different treatments,the comprehensive scores were expressed as calcium nitrate>ammonium nitrate>ammonium sulfate>urea treatment.Nitrate nitrogen and ammonium nitrogen as the only nitrogen source were beneficial to the growth and development of tobacco seedlings,while urea as the only nitrogen source had the worst effects.The findings of this study served as a theoretical foundation and point of reference for the application of various nitrogen sources during the development of flue-cured tobacco seedlings.