139名跨境工作人员淋巴脉络丛脑膜炎病毒和汉坦病毒感染调查分析

Preliminary investigation and risk analysis of the infection with lymphocytic choriomeningitis virus and hantavirus in 139 cross-border migrant workers

目的调查跨境务工人员经鼠传播淋巴脉络丛脑膜炎病毒(lymphocytic choriomeningitis virus, LCMV )和汉坦病毒(hantavirus, HV)的感染状况, 初步评估鼠传病毒感染风险。方法本研究于2019—2020年间, 针对常年在境外从事户外工程工作的务工人员开展调查, 采集血清样本, 通过基于LCMV重组核蛋白的间接ELISA方法、免疫印迹法和基于LCMV重组糖蛋白的间接免疫荧光法检测样品血清中LCMV特异性IgG抗体, 采用检测汉坦病毒IgG抗体的商业化ELISA试剂盒和汉坦病毒感染抗原片检测血清中HV IgG抗体情况。结果共调查了139名跨境工作者, 年龄在25~57岁之间, 64%(89/139)有多个国家工作经历, 涉及26个国家, 包括14个亚洲国家和12个非洲国家, LCMV IgG抗体检出率11.51%(16/139), 阳性样本经免疫印迹法和间接免疫荧光法核实, 抗体检出率略高于类似调查中发现的人群感染率;HV抗体检出率0.72%(1/139), 但尚不能确定获得感染的时间和地区。结论本研究揭示了境外务工人员中LCMV和HV病毒感染状况和存在风险, 提示户外工程场所加强鼠传疾病防控的必要性。

Objective To investigate the prevalence of lymphocytic choriomeningitis virus(LCMV)and hantavirus(HV)specific antibodies among cross-border migrant workers for assessment of the risk of rodents-borne virus infection.Methods From 2019 to 2020,a survey was conducted on cross border migrant workers engaged in outdoor activities,and serum samples were collected,LCMV specific IgG antibody was detected by an indirect ELISA and Western blot based on recombinant nucleoprotein,and indirect immunofluorescence assay(IFA)based on recombinant expressed glycoprotein.HV IgG antibody in serum was detected by a commercial indirect IgG ELISA kit and IFA based on hantavirus infected Vero cells.Results A total of 139 cross-border workers,aged 25~57,were surveyed;64%(89/139)had working experience in multiple countries,involving 26 countries,including 14 countries in Asia and 12 countries in Africa;11.51%(16/139)of serum samples were tested positive for LCMV antibodies,and the positive samples were verified by Western blot and IFA.The antibody detection rate was slightly higher than the published infection rate from other similar studies.And,HV antibodies were detected from one serum sample(0.72%,1/139)by ELISA and IFA.However,it was still uncertain when and where the viral infections were acquired.Conclusions Through this serological cross-sectional preliminary analysis,the infection status and existing risks of LCMV and HV viruses among cross border migrant workers were revealed,which suggested the necessity of strengthening the prevention and control of rodents borne diseases in outdoor engineering sites.