胸膜肺炎放线杆菌分离株血清型的PCR鉴定和Apx毒素基因检测

Molecular Serotyping and Apx Gene Profile Analysis of Actinobacillus pleuropneumoniae Isolates

旨在调查胸膜肺炎放线杆菌流行株的血清型及Apx毒素基因携带情况,采用PCR方法对APP分离株进行血清型鉴定和Apx毒素基因检测。结果表明,64个分离株分别被鉴定为2、5、7、8、12、15、19共7个血清型。7个血清型中发现12种不同的Apx毒力因子模式。毒力因子分析结果显示,同一血清型的不同菌株携带的Apx毒素基因存在差异,不同血清型的菌株也可能会携带相同模式的Apx毒素基因。本研究表明,血清8型胸膜肺炎放线杆菌是浙江省的优势血清型,血清19型菌株在国内首次被检测到。血清型与所携带的Apx毒素基因之间不存在必然联系。

The study aimed to investigate the serotypes and Apx toxin genes of Actinobacillus pleuropneumoniae clinical isolates.PCR was used to identify the serotypes and detect the Apx toxin genes of APP isolates.The results showed that 64 isolates were identified as serotypes 2,5,7,8,12,15,19 respectively.A total of 12 different Apx virulence factor patterns were found in 7 serotypes.The results of virulence factor analysis showed that different strains of the same serotype carried different Apx toxin genes,and the strains of different serotypes may also carry the same pattern of Apx toxin genes.A.pleuropneumoniae serotype 8 was the dominant serotype in Zhejiang Province,and serotype 19 was detected for the first time in China.There is no correlation between the Apx toxin gene and the serotype.