酶促反应结晶研究进展

Research progress of enzymatic reactive crystallization

在“双碳”背景下,绿色可持续的酶促反应正受到工业界的广泛关注,但在实际应用中仍面临着诸多挑战,如反应平衡的限制、不稳定产物的分解、酶的产物抑制等。结晶作为一种高效成熟的分离技术,可通过移除液相产物的方式有效解决上述问题。同时,结晶也是晶体产品的“生成”过程,其与酶促反应耦合可一步实现晶体产品的高效、绿色、可控制备。综述了近年来酶促反应结晶的研究进展,介绍了原位产物结晶(ISPC)技术的发展历程,并讨论了结晶与酶促反应耦合时的相互影响关系;从结晶方式和过程控制角度阐述了酶促反应结晶的实现形式和连续化过程;最后,对酶促反应结晶这一耦合过程的发展和应用进行了总结和展望。

Under the background of“carbon peaking and carbon neutrality”,green and sustainable enzymatic reaction is receiving extensive attention.However,it still faces many challenges in practical application,such as the limitation of reaction equilibrium,the decomposition of unstable products,and the product inhibition of enzyme.As an efficient and mature separation technology,crystallization can effectively solve the above problems by removing liquid phase products.Moreover,crystallization is also the“generation”process of crystals,and the combination of crystallization and enzymatic reaction can realize the efficient,green and controllable preparation of crystal products in one step.This paper reviews the research progress of enzymatic reactive crystallization in recent years.Specifically,the development of in situ product crystallization(ISPC)was introduced,and the interplay between crystallization and enzymatic reaction was discussed.From the perspective of crystallization mode and process control,the realization form and continuous process of enzymatic reactive crystallization were described.Finally,the development and application of this coupled process were summarized and prospected.