一款人参复合配方饮料缓解体力疲劳功能评价及其作用机制研究

Study on the functional mechanism of ginseng compound in relieving physical fatigue

目的评价一款人参复方的缓解体力疲劳功能,并研究其作用机制。方法将250只健康雄性昆明小鼠分成5组,每组50只,分别为对照组、糖对照组、人参复方10倍浓缩液的4.16、8.33、16.66 mL/kg·BW剂量组;连续灌胃30 d后,每组选取20只小鼠进行负重游泳实验,剩余30只分别随机挑选10只小鼠用于血乳酸、肝糖原和力竭游泳实验,基于力竭实验(高尿素模型)开展机制研究,包括受试物对股四头肌抗氧化通路和线粒体自噬通路的影响,以及损伤标志物的变化。结果与对照组相比,人参复方组小鼠更活跃,4.16和16.66 mL/kg·BW剂量组小鼠负重游泳时间显著增加(P<0.05);人参复方3个剂量组的血乳酸曲线下面积显著降低(P<0.05);力竭实验中,4.16 mL/kg·BW组血尿素氮显著降低,3个剂量组骨骼肌核转录因子(Nrf2)转录水平、超氧化物歧化酶和过氧化氢酶活性显著升高(P<0.05),Pten诱导的假定激酶1(PINK1)-E3泛素连接酶(Parkin)转录水平显著降低(P<0.05);3个剂量组血清肌酸苷激酶、乳酸脱氢酶、丙二醛显著降低(P<0.05)。结论人参复方具有缓解体力疲劳的作用,其机制与通过Nrf2/ARE(抗氧化反应原件)-PINK1-Parkin通路减轻力竭所致的骨骼肌线粒体自噬相关。

Objective To evaluate the physical fatigue relief function of ginseng compound formula beverage(ginseng compound)and explore its molecular mechanism.Methods Two hundred and fifty healthy male KM mice were divided into 5 groups with 50 mice in each group,including control group,sugar control group and 10 times concentrated beverage of ginseng compound groups:4.16,8.33 and 16.66 mL/kg·BW.After continuously gavage for 30 d,20 mice for weightbearing swimming test,and the remaining 30 mice in each group were randomly selected for blood lactic acid,liver glycogen and exhaustive swimming experiments,based on the exhaustion experiment(high-urea model)to carry out mechanism exploration,including the effects of test subjects on exhaustion exercise-induced injury,antioxidant pathway and mitochondrial autophagy pathway of quadriceps femoris muscle.Results Compared with the control group,mice in the ginseng compound group were more active,and the weight-bearing swimming time of mice in the 4.16 and 16.66 mL/kg·BW groups increased significantly(P<0.05).The area integral of blood lactic acid decreased significantly in the three dose groups(P<0.05).Blood urea nitrogen of 4.16 mL/kg·BW group in the exhaustion experiment decreased significantly(P<0.05),Nrf2 transcript level in skeletal muscle,activities of superoxide dismutase and catalase increased significantly(P<0.05),PINK1-Parkin transcript level decreased significantly(P<0.05).Creatine kinase,lactate dehydrogenase and malonaldehyde in serum decreased significantly in the three dose groups(P<0.05).Conclusion Ginseng compound has the effect of relieving physical fatigue,and its mechanism may be related to the reduction of skeletal muscle mitophagy caused by exhaustion through the Nrf2/ARE-PINK1-Parkin pathway.