碰撞/反应池技术-电感耦合等离子体质谱法测定食品中硒含量

Determination of Selenium Content in Food by Collision/Reaction Cell Technology Inductively Coupled Plasma Mass Spectrometry

[目的]建立碰撞/反应池技术-电感耦合等离子体质谱法(DRC-ICP-MS)测定食品中硒含量的分析方法。[方法]采用微波消解法,对茶叶、鸡蛋、保健食品、婴幼儿乳粉进行微波消解,于180℃赶酸至近干,定容至50 mL,采用甲烷作为反应气体,以最大丰度80 Se作为质量数,用DRC-ICP-MS测定上述样品中硒的含量。[结果]该方法在0.5~100.0μg/L线性关系良好(R^(2)=0.999949);检出限分别为茶叶、婴幼儿乳粉0.0023 mg/kg,保健食品0.0009 mg/kg,鸡蛋0.0005 mg/kg;加标回收率为93.3%~108.8%,精密度为0.7%~4.7%。应用该方法对茶叶中硒质控样(CFAPA-QC485B-6)中的硒进行了测定,结果符合给定的标准值。[结论]该方法能有效消除^(40)Ar ^(40)Ar对^(80)Se的干扰,提高了检测的灵敏度,并确保了检测结果的准确性,具有灵敏度高、准确性好、线性范围广、检出限低、快速简便等优点。

[Objective]To establish an analytical method for the determination of selenium content in food by collision/reaction cell technology inductively coupled plasma mass spectrometry(DRC-ICP-MS).[Method]Tea,eggs,health food and infant milk powder were digested by microwave digestion method.The acid was driven to nearly dry at 180℃and the volume was fixed to 50 mL.Methane was used as the reaction gas and the maximum abundance of 80 Se was used as the mass number.The content of selenium in the above samples was determined by DRC-ICP-MS.[Result]The linear relationship of 0.5-100.0μg/L concentration range was good(R^(2)=0.999949).The detection limits were tea,infant milk powder 0.0023 mg/kg,health food 0.0009 mg/kg,egg 0.0005 mg/kg.The recovery was 93.3%-108.8%,and the precision was 0.7%-4.7%.The method was applied to the determination of selenium in quality control sample of selenium in tea(CFAPA-QC485B-6),and the results accorded with the given standard value.[Conclusion]This method can effectively eliminate the interference of ^(40) Ar ^(40) Ar to ^(80) Se,improve the sensitivity of detection,and ensure the accuracy of detection results.It has the advantages of high sensitivity,good accuracy,wide linear range,low detection limit,fast and simple.