复合益生菌粉对功能性便秘小鼠润肠通便的作用

The laxative effect of compound probiotic powder in functional constipation mice

目的 探究由乳双歧杆菌V9、干酪乳杆菌Zhang、植物乳杆菌P9组成的复合益生菌粉对便秘模型小鼠的排便情况、肠动力水平的改善及肠道菌群结构的调整作用。方法 将100只Balb/c小鼠随机分为A、B两大组,每组又随机分为空白组、模型组以及复合益生菌粉低剂量组、中剂量组、高剂量组,其中A组50只用于排便情况测定,B组50只用于结肠HE染色及肠道菌群的检测。实验共进行15 d,空白组与模型组小鼠灌胃蒸馏水15 d,复合益生菌粉各剂量组小鼠灌胃相应剂量的复合益生菌粉15 d;同时,除空白组外,于第7天、第15天灌胃盐酸洛哌丁胺(4 mg/kg)进行造模。结果 与空白组相比,模型组小鼠首次排便时间显著延长(P<0.01),6 h内粪便质量显著减轻(P<0.05),肠推进率显著降低(P<0.05),提示造模成功;与模型组相比,复合益生菌粉低、高剂量组首次排黑便时间显著缩短(P<0.05),肠推进率显著升高(P<0.05),同时高剂量组小鼠6 h排便量增加(P<0.05)。HE结果显示,复合益生菌粉可以增厚结肠肌层,恢复受损的结肠黏膜组织,并且腺管排列恢复至正常水平。菌群测序结果显示,与空白组相比,模型组小鼠肠道菌群结构发生显著改变;与模型组相比,复合益生菌粉中、高剂量组显著提高便秘模型小鼠肠道菌群的丰富度,其中Patescibacteria、厚壁菌门、放线菌门、疣微菌门、Eubacterium xylanophilum_group等丰度增加,另枝菌属、Odoribacter等丰度降低。结论 复合益生菌粉能够通过促进肠蠕动,调整肠道菌群结构发挥缓解小鼠便秘的作用。

Objective To explore the effects of compound probiotic powder(CPP) composed of Bifidobacterium animalis subsp. lactis V9, Lactobacillus casei Zhang and Lactobacillus plantarum P9 for defecation, improvement of intestinal motility, and adjustment of intestinal flora structure in constipation model mice. Methods A total of 100 Balb/c mice were randomly divided into group A and group B, and the two groups were further randomly divided into blank control group, model group, low-dose group, medium-dose group, and high-dose group. The 50 mice in group A were used for determination of defecation, while the 50 mice in group B were used for colonic HE staining and intestinal flora detection.The experiment lasted for 15 days;the blank control group and model group were given distilled water, while the CPP groups were given corresponding doses of CPP. Except for the blank control group, the models were established by intragastric administration of loperamide(4 mg/kg) on the 15th days, respectively. Results Compared with the blank control group, the time to first defecation in the model group was longer significantly(P<0.01), the stool weight within 6 hours reduced significantly(P<0.05), and the intestinal propulsion rate was lower significantly(P<0.05), indicating that the model was established successfully. Compared with the model group, the times to first melena excretion in the low-and highdose groups shortened significantly(P<0.05), the intestinal propulsion rates increased significantly(P<0.05), while the stool weights within 6 hours in the high-dose group increased significantly(P<0.05). HE results showed that the CPP thickened the colonic muscle layer, restored the damaged colonic mucosal tissue, and made the arrangement of glandular ducts restore to normal levels. The results of microflora sequencing showed that the structure of intestinal microflora in the model group significantly changed compared with the normal group. Compared with the model group, the richness of intestinal flora of the constipation model mice in the high-dose group significantly improved, with the abundances of Patescibacteria, Firmicutes, Actinomyces, Verrucomicrobia, and Eubacterium xylanophilum_group increased while those of Alistipes and Odoribacter decreased. Conclusion Compound probiotic powder can relieve constipation in mice by promoting intestinal peristalsis and adjusting the structure of intestinal colony.