肝硬化患者与健康人群唾液菌群的对比分析

Comparative analysis of salivary microbiome in liver cirrhosis patients vs healthy population

目的 分析肝硬化患者(LC组)与健康人群(N组)唾液菌群丰度及多样性的差异,为后续研究提供参考。方法 收集45例肝硬化患者和44例健康人群唾液样本,应用高通量测序技术进行测序分析,对有效序列进行物种注释及多样性分析。结果 共获得7 729 352条有效序列,平均每个样本获得有效序列86 847条。两组人群唾液样本内菌群丰度及多样性差异无统计学意义(均P>0.05);而唾液菌群结构差异有统计学意义(F=20.13,P<0.01)。肝硬化患者口腔中厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidota)、梭杆菌门(Fusobacteriota)相对丰度较高,而在健康人群口腔中变形菌门(Proteobacteria)相对丰度较高(均P<0.05)。结论 肝硬化患者与健康人群唾液菌群结构有差异,肝硬化患者相较于健康人群可能更易患龋病、牙周炎。重视口腔健康,防治口腔菌群失调对肝硬化的治疗具有重要意义。

Objective To compare the differences in the abundance and diversity of salivary microbiome between liver cirrhosis patients(LC group) and healthy population(N group). Methods The saliva specimens of 45 LC patients and 44healthy individuals were collected and analyzed using high-throughput sequencing technology, including species annotation and diversity analysis of effective tags. Results A total of 7,729,352 valid sequences were obtained, with an average of 86,847 valid sequences per sample. There were no statistical significances in the abundance and diversity of microbiome between the two groups(all P>0.05), but in the composition of oral microbial community(F = 20.13, P<0.01). The phyla of Firmicutes, Bacteroidota and Fusobacteriota in LC patients showed high abundance, while Proteobacteria showed high abundance in normal population(all P<0.05). Conclusion The structure of oral saliva microbiome in LC patients is different from that in healthy individuals. LC patients may be more prone to caries and periodontitis. Oral health and prevention of oral microbial disorder are of great significance to the diagnosis and treatment of liver cirrhosis.