基于秀丽隐杆线虫痴呆模型的络塞维抗阿尔茨海默病药效研究

Efficacy of Rosavin Against Alzheimer′s Disease:Based on Caenorhabditis elegans(CL4176)Model

目的:基于秀丽隐杆线虫(以下简称线虫)痴呆模型,探究络塞维抗阿尔茨海默病(AD)的作用及可能机制。方法:以转基因线虫CL4176为模型,在显微镜下考察络塞维对线虫瘫痪情况、寿命、运动能力、吞咽频率、活性氧(ROS)水平、抗氧化酶活力等指标的影响。结果:络塞维能够显著降低痴呆模型线虫瘫痪率(P<0.05),延长其寿命,增强其运动能力,显著提高其体内超氧化物歧化酶和过氧化氢酶的活力(P<0.05),显著降低其体内ROS水平和丙二醛含量(P<0.05)。络塞维对模型线虫的吞咽频率没有影响。结论:基于线虫痴呆模型,络塞维有抗阿尔茨海默病的作用,机制可能与改善氧化应激有关。

Objective:To explore the anti-Alzheimer′s disease(AD)effect and possible mechanism of rosavin with Caenorhabditis elegans.Methods:Transgenic C.elegans CL4176 with AD was used,and the effect of rosavin on the paralysis,lifespan,mobility,pharyngeal pumping frequency,reactive oxygen species,and activity of antioxidant enzymes of C.elegans CL4176 was observed under the microscope.Results:Rosavin can reduce the paralysis rate(P<0.05),prolong the lifespan,improve the mobility,increase the activity of superoxide dismutase and catalase(P<0.05),and decrease the reactive oxygen species and malondialdehyde content in the C.elegans CL4176(P<0.05).It had no influence on the pharyngeal pumping rate of the nematodes.Conclusion:It is demonstrated that rosavin showed anti-AD effect on C.elegans model,and the possible mechanism may have to do with improving oxidative stress.