超高效液相色谱-串联质谱法快速检测白酒与配制酒中8种合成甜味剂

Determination of 8 artificial sweeteners in liquor and mixed liquor by UPLC-MS/MS

通过超高效液相色谱-串联质谱法建立快速检测白酒和配制酒中8种合成甜味剂的方法,并对8种甜味剂进行基质效应评价和定性定量分析。结果表明:8种合成甜味剂在10.0~200.0 ng/mL内呈良好线性关系,相关系数R^(2)≥0.999,检出限为1.0~14.3μg/kg,定量限为3.3~47.6μg/kg,加标回收率88.2%~111.4%,相对标准偏差(RSD)1.1%~10.0%;白酒基质效应不明显,配制酒中的8种甜味剂基质效应明显,其中安赛蜜基质增强作用最明显,达300.0%;10份白酒样品中检出1份甜蜜素,含量为0.158 mg/kg, 10份配制酒样品中检出1份安赛蜜,含量为0.258 mg/kg, 1份甜蜜素,含量为0.238 mg/kg。该方法简单、快速、高效、准确度高,可作为白酒和配制酒中8种合成甜味剂的定量检测方法。

The rapid method for the determination of 8 kinds of synthetic sweeteners in liquor and mixed liquor was established by ultra performance liquid chromatography tandem mass spectrometry. Matrix effects of 8 kinds of sweeteners were evaluated and their qualitative and quantitative analysis were carried out. The results showed that the correlation coefficient was R^(2)≥0.999, the detection limits were 1.0-14.3 μg/kg, the limits of quantification were 3.3-47.6 μg/kg, and the recoveries were 88.2 %-111.4 %, relative standard deviation(RSD)1.1 %-10.0 %. The matrix effect of liquor was not obvious, but 8 kinds of sweetener matrix effect was obvious, among which acesulfamil matrix had the most obvious enhancement effect,(reached 300.0 %). One saccharine content of 0.158 mg/kg was detected in 10 liquor samples, one acesulfamil content of 0.258 mg/kg and one saccharine content of 0.238 mg/kg were detected in 10 mixed liquor samples. The method was simple, rapid, efficient and accurate, and could be used for the quantitative determination of 8 kinds of synthetic sweeteners in liquor and mixed liquor.